Restriction map for
pF1KB8538
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BcnI BlnI BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BsrDI
BssHII BssSI Bst1107I BstUI BstZ17I
Bsu36I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRV FauI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflII 976 CTTAAG C^TTAAG
Alw44I 2415 GTGCAC G^TGCAC
ApaLI 2415 GTGCAC G^TGCAC
AseI 1464 ATTAAT AT^TAAT
AsnI 1464 ATTAAT AT^TAAT
AvaII 9 GGACC G^G[AT]CC
BanI 472 GGTGCC G^G[CT][GA]CC
BciVI 150 GTATCC GTATCC(6/5)
BfrI 976 CTTAAG C^TTAAG
BglI 2206 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1767 AGATCT A^GATCT
BlpI 1060 GCTCAGC GC^T[ACGT]AGC
BpmI 1928 CTCCAG CTGGAG(16/14)
Bpu1102I 1060 GCTCAGC GC^T[ACGT]AGC
BsaAI 1449 CACGTA [CT]AC^GT[GA]
BsaBI 1762 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 596 GGTCTC GGTCTC(1/5)
BsiEI 1045 CGATCG CG[GA][CT]^CG
BsmI 2461 GCATTC GAATGC(1/-1)
Bsp1407I 1558 TGTACA T^GTACA
BspMI 1634 ACCTGC ACCTGC(4/8)
BsrFI 11 ACCGGC [GA]^CCGG[CT]
BsrGI 1558 TGTACA T^GTACA
BstBI 758 TTCGAA TT^CGAA
BstEII 2098 GGTGACC G^GT[ACGT]ACC
BstPI 2098 GGTGACC G^GT[ACGT]ACC
BstXI 2231 CCATTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1060 GCTCAGC GC^T[ACGT]AGC
CfoI 1485 GCGC GCG^C
Cfr10I 11 ACCGGC [GA]^CCGG[CT]
DraIII 1423 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2466 CCGTGG C^C[GA][CT]GG
EaeI 1030 CGGCCA [CT]^GGCC[GA]
EcoO65I 2098 GGTGACC G^GT[ACGT]ACC
EcoRI 2182 GAATTC G^AATTC
EcoT14I 380 CCAAGG C^C[AT][AT]GG
EcoT22I 784 ATGCAT ATGCA^T
HapII 12 CCGG C^CGG
HgaI 2670 GCGTC GACGC(5/10)
HhaI 1485 GCGC GCG^C
HinP1I 1485 GCGC G^CGC
HindIII 2111 AAGCTT A^AGCTT
HpaII 12 CCGG C^CGG
MamI 1762 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2232 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 12 CCGG C^CGG
NsiI 784 ATGCAT ATGCA^T
NspV 758 TTCGAA TT^CGAA
PshBI 1464 ATTAAT AT^TAAT
PvuI 1045 CGATCG CGAT^CG
ScaI 950 AGTACT AGT^ACT
SfuI 758 TTCGAA TT^CGAA
SspBI 1558 TGTACA T^GTACA
StyI 380 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 1413 CCGC CCGC(-3/-1)
1441 CCGC
AspHI 734 GAGCAC G[AT]GC[AT]^C
2415 GTGCAC
BbsI 994 GAAGAC GAAGAC(2/6)
1875 GAAGAC
BclI 362 TGATCA T^GATCA
2483 TGATCA
BfaI 1543 CTAG C^TAG
1867 CTAG
BpuAI 994 GAAGAC GAAGAC(2/6)
1875 GAAGAC
BsiHKAI 734 GAGCAC G[AT]GC[AT]^C
2415 GTGCAC
BspHI 545 TCATGA T^CATGA
1248 TCATGA
BspLU11I 1745 ACATGT A^CATGT
2576 ACATGT
BstAPI 63 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
466 GCATTTGGTGC
BstYI 1767 AGATCT [GA]^GATC[CT]
2588 AGATCC
Cfr13I 9 GGACC G^G[ACGT]CC
2382 GGCCC
EcoNI 972 CCTACTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1542 CCTAGAAGAGG
FbaI 362 TGATCA T^GATCA
2483 TGATCA
MaeI 1543 CTAG C^TAG
1867 CTAG
MflI 1767 AGATCT [GA]^GATC[CT]
2588 AGATCC
MspA1I 73 CAGCTG C[AC]G^C[GT]G
2005 CAGCTG
PvuII 73 CAGCTG CAG^CTG
2005 CAGCTG
RcaI 545 TCATGA T^CATGA
1248 TCATGA
Sau96I 9 GGACC G^G[ACGT]CC
2382 GGCCC
SphI 1307 GCATGC GCATG^C
2048 GCATGC
SspI 316 AATATT AAT^ATT
2157 AATATT
Tsp45I 323 GTGAC ^GT[GC]AC
2099 GTGAC
XhoII 1767 AGATCT [GA]^GATC[CT]
2588 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI Asp700I BbvI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MfeI MnlI MseI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI