##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MflI        MluI        MroI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          346  GCGG                 CCGC(-3/-1)
AfaI          167  GTAC                 GT^AC
Alw44I        372  GTGCAC               G^TGCAC
AlwI          268  GGATC                GGATC(4/5)
ApaLI         372  GTGCAC               G^TGCAC
AspHI         372  GTGCAC               G[AT]GC[AT]^C
BalI          107  TGGCCA               TGG^CCA
BanI           19  GGCACC               G^G[CT][GA]CC
BclI          122  TGATCA               T^GATCA
BsaJI          15  CCTGGG               C^C[ACGT][ACGT]GG
BsgI          283  GTGCAG               GTGCAG(16/14)
BsiHKAI       372  GTGCAC               G[AT]GC[AT]^C
BsrI           96  ACTGG                ACTGG(1/-1)
BstF5I        294  GGATG                GGATG(2/0)
BstXI          89  CCAGAAAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          172  TTTAAA               TTT^AAA
EaeI          107  TGGCCA               [CT]^GGCC[GA]
FbaI          122  TGATCA               T^GATCA
FokI          294  GGATG                GGATG(9/13)
MaeIII        253  GTAAC                ^GT[ACGT]AC
MboII         214  GAAGA                GAAGA(8/7)
MluNI         107  TGGCCA               TGG^CCA
MnlI          266  GAGG                 CCTC(7/6)
MscI          107  TGGCCA               TGG^CCA
MseI          173  TTAA                 T^TAA
MspA1I        224  CAGCTG               C[AC]G^C[GT]G
MwoI          365  GCGTGGTGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII         60  CATG                 CATG^
NlaIV          19  GGCACC               GG[ACGT]^[ACGT]CC
PstI          259  CTGCAG               CTGCA^G
PvuII         224  CAGCTG               CAG^CTG
RsaI          167  GTAC                 GT^AC
SfaNI         248  GCATC                GCATC(5/9)
SfcI          259  CTGCAG               C^T[GA][CT]AG
Tru9I         173  TTAA                 T^TAA
XcmI           86  CCACCAGAAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BmyI           18  GGGCAC               G[AGT]GC[ACT]^C
              372  GTGCAC              
BsmAI         304  GAGAC                GTCTC(1/5)
               34  GTCTC               
Bsp1286I       18  GGGCAC               G[AGT]GC[ACT]^C
              372  GTGCAC              
DpnII         123  GATC                 ^GATC
              269  GATC                
HaeIII        108  GGCC                 GG^CC
              202  GGCC                
HinfI         276  GAATC                G^A[ACGT]TC
              380  GAATC               
MboI          123  GATC                 ^GATC
              269  GATC                
NdeII         123  GATC                 ^GATC
              269  GATC                
Sau3AI        123  GATC                 ^GATC
              269  GATC                
TfiI          276  GAATC                G^A[AT]TC
              380  GAATC               
TspRI         117  CACTG                CA[GC]TG[ACGT][ACGT]^
              272  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        BssKI       
BstNI       EcoRII      MaeI        MvaI        ScrFI       
Tsp509I