Restriction map for
pF1KB7996
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BciVI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MslI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tsp509I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Aor51HI 789 AGCGCT AGC^GCT
AspI 1353 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 272 TGGCCA TGG^CCA
BbeI 202 GGCGCC GGCGC^C
BbvCI 792 GCTGAGG CCTCAGC(-5/-2)
BcgI 830 CGAAGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1316 TGATCA T^GATCA
BfaI 640 CTAG C^TAG
BlpI 384 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 384 GCTGAGC GC^T[ACGT]AGC
BsaWI 896 ACCGGA [AT]^CCGG[AT]
BsiEI 315 CGACCG CG[GA][CT]^CG
BsmBI 556 GAGACG CGTCTC(1/5)
CelII 384 GCTGAGC GC^T[ACGT]AGC
EarI 595 CTCTTC CTCTTC(1/4)
Eco47III 789 AGCGCT AGC^GCT
EcoT14I 242 CCATGG C^C[AT][AT]GG
FbaI 1316 TGATCA T^GATCA
KasI 202 GGCGCC G^GCGCC
Ksp632I 595 CTCTTC CTCTTC(1/4)
MaeI 640 CTAG C^TAG
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MseI 1249 TTAA T^TAA
MspA1I 424 CAGCTG C[AC]G^C[GT]G
NarI 202 GGCGCC GG^CGCC
NcoI 242 CCATGG C^CATGG
NlaIII 243 CATG CATG^
PaeR7I 1081 CTCGAG C^TCGAG
PflFI 1353 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 521 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 424 CAGCTG CAG^CTG
SapI 594 GCTCTTC GCTCTTC(1/4)
SfoI 202 GGCGCC GGC^GCC
StyI 242 CCATGG C^C[AT][AT]GG
Tru9I 1249 TTAA T^TAA
TspRI 1377 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1353 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1081 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 202 GGCGCC G[GA]^CG[CT]C
947 GACGCC
AlwI 954 GATCC GGATC(4/5)
1217 GGATC
AlwNI 295 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
436 CAGGAGCTG
AocI 1125 CCTGAGG CC^T[ACGT]AGG
1270 CCTGAGG
BanI 202 GGCGCC G^G[CT][GA]CC
1121 GGCACC
BbsI 1305 GAAGAC GAAGAC(2/6)
1392 GTCTTC
BglI 131 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1042 GCCCTGGAGGC
BpuAI 1305 GAAGAC GAAGAC(2/6)
1392 GTCTTC
BsaHI 202 GGCGCC G[GA]^CG[CT]C
947 GACGCC
BsaI 710 GAGACC GGTCTC(1/5)
1330 GAGACC
BsrBI 191 GAGCGG CCGCTC(-3/-3)
197 GAGCGG
BsrFI 28 GCCGGC [GA]^CCGG[CT]
408 GCCGGC
BstAPI 294 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
435 GCAGGAGCTGC
BstF5I 801 CATCC GGATG(2/0)
666 GGATG
BstYI 953 AGATCC [GA]^GATC[CT]
1217 GGATCT
Bsu36I 1125 CCTGAGG CC^T[ACGT]AGG
1270 CCTGAGG
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
408 GCCGGC
DsaI 242 CCATGG C^C[GA][CT]GG
764 CCACGG
EaeI 245 TGGCCG [CT]^GGCC[GA]
272 TGGCCA
Eco81I 1125 CCTGAGG CC^T[ACGT]AGG
1270 CCTGAGG
FokI 801 CATCC GGATG(9/13)
666 GGATG
Hin1I 202 GGCGCC G[GA]^CG[CT]C
947 GACGCC
MflI 953 AGATCC [GA]^GATC[CT]
1217 GGATCT
NaeI 28 GCCGGC GCC^GGC
408 GCCGGC
NgoMIV 28 GCCGGC G^CCGGC
408 GCCGGC
SbfI 714 CCTGCAGG CCTGCA^GG
837 CCTGCAGG
SfaNI 616 GATGC GCATC(5/9)
821 GCATC
SmaI 85 CCCGGG CCC^GGG
395 CCCGGG
Sse8387I 714 CCTGCAGG CCTGCA^GG
837 CCTGCAGG
Tsp45I 485 GTGAC ^GT[GC]AC
1351 GTGAC
XhoII 953 AGATCC [GA]^GATC[CT]
1217 GGATCT
XmaCI 85 CCCGGG C^CCGGG
395 CCCGGG
XmaI 85 CCCGGG C^CCGGG
395 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BssHII BssKI
BssSI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfcI SmlI
TaqI TseI TthHB8I