Restriction map for
pF1KE1038
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwNI 257 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 232 CCTCAGG CC^T[ACGT]AGG
AseI 1000 ATTAAT AT^TAAT
AsnI 1000 ATTAAT AT^TAAT
Asp700I 826 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 511 GTGCTC G[AT]GC[AT]^C
AvaI 184 CCCGGG C^[CT]CG[GA]G
BanI 1337 GGTGCC G^G[CT][GA]CC
BanII 958 GAGCCC G[GA]GC[CT]^C
BglI 1388 GCCAGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 713 GAGGAG GAGGAG(10/8)
BsiHKAI 511 GTGCTC G[AT]GC[AT]^C
BsmFI 628 GGGAC GGGAC(10/14)
BsoBI 184 CCCGGG C^[CT]CG[GA]G
Bsu36I 232 CCTCAGG CC^T[ACGT]AGG
Cac8I 496 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 649 GCGC GCG^C
EarI 1951 GAAGAG CTCTTC(1/4)
Eco81I 232 CCTCAGG CC^T[ACGT]AGG
EcoNI 1603 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1424 GAATTC G^AATTC
HapII 185 CCGG C^CGG
HgaI 863 GCGTC GACGC(5/10)
HhaI 649 GCGC GCG^C
HinP1I 649 GCGC G^CGC
HpaII 185 CCGG C^CGG
Ksp632I 1951 GAAGAG CTCTTC(1/4)
MfeI 1379 CAATTG C^AATTG
MslI 391 CATTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1290 CAGCTG C[AC]G^C[GT]G
MspI 185 CCGG C^CGG
MunI 1379 CAATTG C^AATTG
NdeI 1409 CATATG CA^TATG
PshBI 1000 ATTAAT AT^TAAT
PvuII 1290 CAGCTG CAG^CTG
SmaI 184 CCCGGG CCC^GGG
XcmI 676 CCAGTACGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 184 CCCGGG C^CCGGG
XmaI 184 CCCGGG C^CCGGG
XmnI 826 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1449 CCGC CCGC(-3/-1)
599 GCGG
AlwI 3 GGATC GGATC(4/5)
529 GGATC
BclI 843 TGATCA T^GATCA
1500 TGATCA
BcnI 184 CCCGG CC^[GC]GG
185 CCGGG
BfaI 1648 CTAG C^TAG
1948 CTAG
BmyI 511 GTGCTC G[AGT]GC[ACT]^C
958 GAGCCC
BsgI 1991 CTGCAC GTGCAG(16/14)
1867 GTGCAG
Bsp1286I 511 GTGCTC G[AGT]GC[ACT]^C
958 GAGCCC
BspMI 1989 ACCTGC ACCTGC(4/8)
33 GCAGGT
BstF5I 668 CATCC GGATG(2/0)
1722 CATCC
BstYI 3 GGATCT [GA]^GATC[CT]
529 GGATCT
FbaI 843 TGATCA T^GATCA
1500 TGATCA
FokI 668 CATCC GGATG(9/13)
1722 CATCC
MaeI 1648 CTAG C^TAG
1948 CTAG
MflI 3 GGATCT [GA]^GATC[CT]
529 GGATCT
NciI 184 CCCGG CC^[GC]GG
185 CCGGG
NlaIV 562 GGACCC GG[ACGT]^[ACGT]CC
1337 GGTGCC
PstI 31 CTGCAG CTGCA^G
254 CTGCAG
SmlI 78 CTCAAG C^T[CT][GA]AG
1366 CTCAAG
XbaI 1647 TCTAGA T^CTAGA
1947 TCTAGA
XhoII 3 GGATCT [GA]^GATC[CT]
529 GGATCT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BbvI BpmI BpuAI BsaJI
BsiYI BslI BspHI BsrI BssKI
BstNI Cfr13I DdeI DpnII DraI
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI