Restriction map for
pF1KB6547
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AlwI
AlwNI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MflI MluI MroI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AflII 988 CTTAAG C^TTAAG
AflIII 435 ACATGT A^C[GA][CT]GT
Alw44I 146 GTGCAC G^TGCAC
AocI 616 CCTGAGG CC^T[ACGT]AGG
ApaLI 146 GTGCAC G^TGCAC
AseI 660 ATTAAT AT^TAAT
AsnI 660 ATTAAT AT^TAAT
Asp700I 136 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 47 CTCGAG C^[CT]CG[GA]G
BalI 447 TGGCCA TGG^CCA
BanII 938 GAGCTC G[GA]GC[CT]^C
BbvI 801 GCTGC GCAGC(8/12)
BclI 969 TGATCA T^GATCA
BfaI 904 CTAG C^TAG
BfrI 988 CTTAAG C^TTAAG
BsaAI 754 TACGTG [CT]AC^GT[GA]
BseRI 416 CTCCTC GAGGAG(10/8)
BsoBI 47 CTCGAG C^[CT]CG[GA]G
Bsp1407I 154 TGTACA T^GTACA
BspLU11I 435 ACATGT A^CATGT
BspMI 513 ACCTGC ACCTGC(4/8)
BsrGI 154 TGTACA T^GTACA
BssKI 1029 CCAGG ^CC[ACGT]GG
BstAPI 1087 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1029 CCAGG CC^[AT]GG
Bsu36I 616 CCTGAGG CC^T[ACGT]AGG
Cac8I 367 GCAGGC GC[ACGT]^[ACGT]GC
DraI 636 TTTAAA TTT^AAA
EaeI 447 TGGCCA [CT]^GGCC[GA]
Eco81I 616 CCTGAGG CC^T[ACGT]AGG
EcoRII 1029 CCAGG ^CC[AT]GG
EcoRV 115 GATATC GAT^ATC
FbaI 969 TGATCA T^GATCA
MaeI 904 CTAG C^TAG
MaeIII 41 GTAAC ^GT[ACGT]AC
MfeI 875 CAATTG C^AATTG
MluNI 447 TGGCCA TGG^CCA
MscI 447 TGGCCA TGG^CCA
MslI 421 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 875 CAATTG C^AATTG
MvaI 1029 CCAGG CC^[AT]GG
NspI 435 ACATGT [GA]CATG^[CT]
PaeR7I 47 CTCGAG C^TCGAG
PflMI 580 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 50 GAGTC GAGTC(4/5)
PshBI 660 ATTAAT AT^TAAT
SacI 938 GAGCTC GAGCT^C
ScrFI 1029 CCAGG CC^[ACGT]GG
SfcI 809 CTATAG C^T[GA][CT]AG
SspBI 154 TGTACA T^GTACA
TfiI 243 GATTC G^A[AT]TC
TseI 801 GCTGC G^C[AT]GC
TspRI 149 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 580 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 47 CTCGAG C^TCGAG
XmnI 136 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 146 GTGCAC G[AT]GC[AT]^C
938 GAGCTC
BbsI 670 GAAGAC GAAGAC(2/6)
52 GTCTTC
BmyI 146 GTGCAC G[AGT]GC[ACT]^C
938 GAGCTC
BpuAI 670 GAAGAC GAAGAC(2/6)
52 GTCTTC
BsiHKAI 146 GTGCAC G[AT]GC[AT]^C
938 GAGCTC
BsmAI 254 GAGAC GTCTC(1/5)
781 GAGAC
BsmFI 274 GGGAC GGGAC(10/14)
12 GTCCC
Bsp1286I 146 GTGCAC G[AGT]GC[ACT]^C
938 GAGCTC
Fnu4HI 371 GCCGC GC^[ACGT]GC
801 GCTGC
HaeIII 370 GGCC GG^CC
448 GGCC
HinfI 50 GAGTC G^A[ACGT]TC
243 GATTC
HphI 298 GGTGA GGTGA(8/7)
505 GGTGA
ItaI 371 GCCGC GC^[ACGT]GC
801 GCTGC
MaeII 62 ACGT A^CGT
755 ACGT
SfaNI 209 GATGC GCATC(5/9)
622 GATGC
SspI 573 AATATT AAT^ATT
832 AATATT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsiYI BslI BsrI BstF5I DdeI
DpnII FokI MboI MboII MnlI
MseI MwoI NdeII NlaIII RsaI
Sau3AI SmlI TaqI Tru9I Tsp509I
TthHB8I