Restriction map for
pF1KB6512
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 881 CCGC CCGC(-3/-1)
AvaII 322 GGTCC G^G[AT]CC
BbsI 950 GAAGAC GAAGAC(2/6)
BbvI 1110 GCAGC GCAGC(8/12)
BcnI 917 CCCGG CC^[GC]GG
BpuAI 950 GAAGAC GAAGAC(2/6)
BsaJI 797 CCCTGG C^C[ACGT][ACGT]GG
BseRI 739 GAGGAG GAGGAG(10/8)
BsiYI 328 CCAGGTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 328 CCAGGTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 241 GCATTC GAATGC(1/-1)
Bsp1407I 416 TGTACA T^GTACA
BsrGI 416 TGTACA T^GTACA
BstAPI 436 GCATTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 716 CATCC GGATG(2/0)
DraII 172 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 904 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 172 AGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 716 CATCC GGATG(9/13)
HapII 918 CCGG C^CGG
HpaII 918 CCGG C^CGG
MspI 918 CCGG C^CGG
NciI 917 CCCGG CC^[GC]GG
PflMI 328 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 1120 CTACAG C^T[GA][CT]AG
SspBI 416 TGTACA T^GTACA
StuI 1020 AGGCCT AGG^CCT
TaqI 138 TCGA T^CGA
TseI 1110 GCAGC G^C[AT]GC
TthHB8I 138 TCGA T^CGA
Van91I 328 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 968 CCAAGTCCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 59 GTAGAC GT^[AC][GT]AC
1000 GTCTAC
AfaI 417 GTAC GT^AC
933 GTAC
AhdI 424 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
814 GACCTGGTGTC
AlwNI 171 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
428 CAGAGTCTG
AspEI 424 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
814 GACCTGGTGTC
BamHI 301 GGATCC G^GATCC
862 GGATCC
BanII 794 GAGCCC G[GA]GC[CT]^C
1076 GGGCTC
BbvCI 1096 CCTCAGC CCTCAGC(-5/-2)
736 GCTGAGG
BfaI 886 CTAG C^TAG
1114 CTAG
BsmAI 1036 GAGAC GTCTC(1/5)
822 GTCTC
BsmFI 840 GTCCC GGGAC(10/14)
972 GTCCC
BstXI 383 CCACCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCATCTGAGTGG
Cfr13I 173 GGGCC G^G[ACGT]CC
322 GGTCC
Eam1105I 424 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
814 GACCTGGTGTC
Fnu4HI 880 GCCGC GC^[ACGT]GC
1110 GCAGC
HaeIII 174 GGCC GG^CC
1021 GGCC
ItaI 880 GCCGC GC^[ACGT]GC
1110 GCAGC
MaeI 886 CTAG C^TAG
1114 CTAG
MseI 491 TTAA T^TAA
611 TTAA
MspA1I 827 CAGCTG C[AC]G^C[GT]G
867 CAGCTG
PvuII 827 CAGCTG CAG^CTG
867 CAGCTG
RsaI 417 GTAC GT^AC
933 GTAC
Sau96I 173 GGGCC G^G[ACGT]CC
322 GGTCC
SexAI 327 ACCAGGT A^CC[AT]GGT
815 ACCTGGT
Tru9I 491 TTAA T^TAA
611 TTAA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BmyI BpmI Bsp1286I
BsrI BssKI BstNI BstYI DdeI
DpnII EcoRII HinfI HphI MaeIII
MboI MboII MflI MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI SfaNI TfiI
Tsp45I Tsp509I TspRI XhoII