Restriction map for
pF1KB6574
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsiEI
BsiHKAI BsiWI BsmFI BsoBI Bsp1286I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI HincII HindII
HpaI KasI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 459 GACGTC GACGT^C
Acc65I 139 GGTACC G^GTACC
AccI 670 GTAGAC GT^[AC][GT]AC
AccIII 556 TCCGGA T^CCGGA
AclI 66 AACGTT AA^CGTT
AcyI 459 GACGTC G[GA]^CG[CT]C
AlwI 972 GATCC GGATC(4/5)
Asp700I 64 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 139 GGTACC G^GTACC
BcnI 907 CCGGG CC^[GC]GG
BglI 648 GCCTAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 459 GACGTC G[GA]^CG[CT]C
BsaI 508 GGTCTC GGTCTC(1/5)
BsaJI 59 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 556 TCCGGA [AT]^CCGG[AT]
BseAI 556 TCCGGA T^CCGGA
BseRI 607 GAGGAG GAGGAG(10/8)
BsgI 841 GTGCAG GTGCAG(16/14)
BsmBI 457 GAGACG CGTCTC(1/5)
BspEI 556 TCCGGA T^CCGGA
BssHII 20 GCGCGC G^CGCGC
BssSI 25 CTCGTG CACGAG(-5/-1)
BstEII 1168 GGTAACC G^GT[ACGT]ACC
BstF5I 810 CATCC GGATG(2/0)
BstPI 1168 GGTAACC G^GT[ACGT]ACC
BstYI 971 AGATCC [GA]^GATC[CT]
DraII 1028 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 600 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1028 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1168 GGTAACC G^GT[ACGT]ACC
FokI 810 CATCC GGATG(9/13)
HaeII 329 GGCGCT [GA]GCGC^[CT]
HaeIII 1030 GGCC GG^CC
Hin1I 459 GACGTC G[GA]^CG[CT]C
HindIII 900 AAGCTT A^AGCTT
HphI 869 TCACC GGTGA(8/7)
KpnI 139 GGTACC GGTAC^C
MflI 971 AGATCC [GA]^GATC[CT]
MroI 556 TCCGGA T^CCGGA
MseI 47 TTAA T^TAA
MslI 223 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 907 CCGGG CC^[GC]GG
NdeI 549 CATATG CA^TATG
PflMI 44 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1089 GAGTC GAGTC(4/5)
Psp1406I 66 AACGTT AA^CGTT
SexAI 1172 ACCAGGT A^CC[AT]GGT
SmlI 337 CTTGAG C^T[CT][GA]AG
SphI 952 GCATGC GCATG^C
TaqI 875 TCGA T^CGA
TfiI 568 GAATC G^A[AT]TC
Tru9I 47 TTAA T^TAA
TthHB8I 875 TCGA T^CGA
Van91I 44 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 971 AGATCC [GA]^GATC[CT]
XmnI 64 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 21 CGCG CG^CG
307 CGCG
AciI 306 CCGC CCGC(-3/-1)
846 GCGG
AlwNI 754 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
970 CAGATCCTG
AvaII 53 GGACC G^G[AT]CC
1176 GGTCC
BanI 139 GGTACC G^G[CT][GA]CC
685 GGCACC
BsmAI 457 GAGAC GTCTC(1/5)
509 GTCTC
BsmI 316 GAATGC GAATGC(1/-1)
613 GCATTC
Bsp1407I 160 TGTACA T^GTACA
1130 TGTACA
BsrGI 160 TGTACA T^GTACA
1130 TGTACA
BstUI 21 CGCG CG^CG
307 CGCG
DpnII 807 GATC ^GATC
972 GATC
HapII 557 CCGG C^CGG
907 CCGG
HinfI 568 GAATC G^A[ACGT]TC
1089 GAGTC
HpaII 557 CCGG C^CGG
907 CCGG
MboI 807 GATC ^GATC
972 GATC
MspI 557 CCGG C^CGG
907 CCGG
MvnI 21 CGCG CG^CG
307 CGCG
NdeII 807 GATC ^GATC
972 GATC
NlaIII 859 CATG CATG^
953 CATG
NspI 858 ACATGC [GA]CATG^[CT]
952 GCATGC
PshAI 124 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
232 GACGAGTGTC
PstI 246 CTGCAG CTGCA^G
268 CTGCAG
Sau3AI 807 GATC ^GATC
972 GATC
SspBI 160 TGTACA T^GTACA
1130 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BbsI BbvI BpuAI
BsiYI BslI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI HhaI HinP1I ItaI MaeII
MaeIII MboII MnlI MspA1I MvaI
MwoI NlaIV PvuII RsaI Sau96I
ScrFI SfcI TseI Tsp45I Tsp509I
TspRI