Restriction map for
pF1KB5775
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbvCI BcgI BciVI BglII
BlnI BseAI BsgI BsiWI BspDI
BspEI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRV FseI
FspI HincII HindII HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 1441 ACGTGT A^C[GA][CT]GT
AlwNI 25 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2195 CCTCAGG CC^T[ACGT]AGG
AvaI 145 CCCGAG C^[CT]CG[GA]G
BanII 127 GAGCTC G[GA]GC[CT]^C
BbrPI 1440 CACGTG CAC^GTG
BclI 1802 TGATCA T^GATCA
BglI 67 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 120 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTCAGC GC^T[ACGT]AGC
BsaAI 1440 CACGTG [CT]AC^GT[GA]
BsaBI 1885 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 808 GAGACC GGTCTC(1/5)
BsaWI 1833 ACCGGA [AT]^CCGG[AT]
BsmBI 663 CGTCTC CGTCTC(1/5)
BsmI 1473 GCATTC GAATGC(1/-1)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1290 TGTACA T^GTACA
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BsrGI 1290 TGTACA T^GTACA
BstBI 1616 TTCGAA TT^CGAA
BstXI 1470 CCAGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2195 CCTCAGG CC^T[ACGT]AGG
CelII 120 GCTCAGC GC^T[ACGT]AGC
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
DraI 1509 TTTAAA TTT^AAA
EarI 1635 GAAGAG CTCTTC(1/4)
Eco81I 2195 CCTCAGG CC^T[ACGT]AGG
EcoNI 132 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 595 GAATTC G^AATTC
FbaI 1802 TGATCA T^GATCA
HgaI 662 GCGTC GACGC(5/10)
HindIII 1457 AAGCTT A^AGCTT
Ksp632I 1635 GAAGAG CTCTTC(1/4)
MamI 1885 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1210 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 76 GCCGGC GCC^GGC
NcoI 2238 CCATGG C^CATGG
NgoMIV 76 GCCGGC G^CCGGC
NspV 1616 TTCGAA TT^CGAA
PmaCI 1440 CACGTG CAC^GTG
PmlI 1440 CACGTG CAC^GTG
PpuMI 14 GGGACCC [GA]G^G[AT]CC[CT]
SacI 127 GAGCTC GAGCT^C
SbfI 327 CCTGCAGG CCTGCA^GG
ScaI 2039 AGTACT AGT^ACT
SfuI 1616 TTCGAA TT^CGAA
Sse8387I 327 CCTGCAGG CCTGCA^GG
SspBI 1290 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 81 CGCG CG^CG
190 CGCG
AflII 235 CTTAAG C^TTAAG
1741 CTTAAG
Asp700I 349 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1230 GAACAGCTTC
BbeI 141 GGCGCC GGCGC^C
194 GGCGCC
BbsI 2136 GAAGAC GAAGAC(2/6)
2014 GTCTTC
BfrI 235 CTTAAG C^TTAAG
1741 CTTAAG
BpuAI 2136 GAAGAC GAAGAC(2/6)
2014 GTCTTC
BseRI 162 GAGGAG GAGGAG(10/8)
2201 GAGGAG
BsiEI 41 CGGCCG CG[GA][CT]^CG
74 CGGCCG
BsmFI 14 GGGAC GGGAC(10/14)
1243 GTCCC
BspHI 1716 TCATGA T^CATGA
1799 TCATGA
BstUI 81 CGCG CG^CG
190 CGCG
BstYI 1119 GGATCT [GA]^GATC[CT]
1950 GGATCT
DraII 14 GGGACCC [GA]G^G[ACGT]CC[CT]
26 AGGCCCT
DsaI 50 CCACGG C^C[GA][CT]GG
2238 CCATGG
EaeI 41 CGGCCG [CT]^GGCC[GA]
74 CGGCCG
EagI 41 CGGCCG C^GGCCG
74 CGGCCG
EclXI 41 CGGCCG C^GGCCG
74 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
74 CGGCCG
EcoO109I 14 GGGACCC [GA]G^G[ACGT]CC[CT]
26 AGGCCCT
EcoT22I 2116 ATGCAT ATGCA^T
2318 ATGCAT
HaeII 141 GGCGCC [GA]GCGC^[CT]
194 GGCGCC
KasI 141 GGCGCC G^GCGCC
194 GGCGCC
MaeII 1441 ACGT A^CGT
1859 ACGT
MflI 1119 GGATCT [GA]^GATC[CT]
1950 GGATCT
MvnI 81 CGCG CG^CG
190 CGCG
NarI 141 GGCGCC GG^CGCC
194 GGCGCC
NsiI 2116 ATGCAT ATGCA^T
2318 ATGCAT
NspI 540 GCATGT [GA]CATG^[CT]
2114 ACATGC
PflMI 975 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2233 CCATTCCATGG
PstI 328 CTGCAG CTGCA^G
2186 CTGCAG
RcaI 1716 TCATGA T^CATGA
1799 TCATGA
SfoI 141 GGCGCC GGC^GCC
194 GGCGCC
SmlI 235 CTTAAG C^T[CT][GA]AG
1741 CTTAAG
SspI 1420 AATATT AAT^ATT
1670 AATATT
Van91I 975 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2233 CCATTCCATGG
XbaI 1608 TCTAGA T^CTAGA
1881 TCTAGA
XcmI 1385 CCATTCCAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2250 CCATTCACATCCTGG
XhoII 1119 GGATCT [GA]^GATC[CT]
1950 GGATCT
XmnI 349 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1230 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I