##################################
# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BseAI
BseRI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tsp45I Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 348 ACGTGT A^C[GA][CT]GT
AgeI 34 ACCGGT A^CCGGT
AlwNI 205 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 316 GAAGAC GAAGAC(2/6)
BbvI 262 GCAGC GCAGC(8/12)
BfaI 379 CTAG C^TAG
BglII 243 AGATCT A^GATCT
BpmI 98 CTGGAG CTGGAG(16/14)
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaAI 392 TACGTG [CT]AC^GT[GA]
BsaJI 49 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 34 ACCGGT [AT]^CCGG[AT]
BsiEI 32 CGACCG CG[GA][CT]^CG
BsrBI 74 CCGCTC CCGCTC(-3/-3)
BsrFI 34 ACCGGT [GA]^CCGG[CT]
BstF5I 372 CATCC GGATG(2/0)
BstYI 243 AGATCT [GA]^GATC[CT]
Cfr10I 34 ACCGGT [GA]^CCGG[CT]
DdeI 227 CTCAG C^T[ACGT]AG
DpnII 244 GATC ^GATC
DraI 235 TTTAAA TTT^AAA
FauI 23 CCCGC CCCGC(4/6)
FokI 372 CATCC GGATG(9/13)
HaeII 141 GGCGCT [GA]GCGC^[CT]
HapII 35 CCGG C^CGG
HgaI 207 GCGTC GACGC(5/10)
HinfI 186 GATTC G^A[ACGT]TC
HpaII 35 CCGG C^CGG
HphI 105 GGTGA GGTGA(8/7)
MaeI 379 CTAG C^TAG
MboI 244 GATC ^GATC
MflI 243 AGATCT [GA]^GATC[CT]
MseI 236 TTAA T^TAA
MspI 35 CCGG C^CGG
NdeII 244 GATC ^GATC
NheI 378 GCTAGC G^CTAGC
NlaIII 340 CATG CATG^
PflMI 204 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 34 ACCGGT A^CCGGT
Sau3AI 244 GATC ^GATC
TaqI 31 TCGA T^CGA
TfiI 186 GATTC G^A[AT]TC
Tru9I 236 TTAA T^TAA
TseI 262 GCAGC G^C[AT]GC
TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 31 TCGA T^CGA
Van91I 204 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 50 CCAGGAAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 243 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 8 GTAC GT^AC
134 GTAC
AluI 264 AGCT AG^CT
377 AGCT
BsiYI 91 CCCTCTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCGTCTGG
BslI 91 CCCTCTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
204 CCAGCGTCTGG
BsrI 60 ACTGG ACTGG(1/-1)
97 ACTGG
BssKI 50 CCAGG ^CC[ACGT]GG
130 CCTGG
BstNI 50 CCAGG CC^[AT]GG
130 CCTGG
EcoRII 50 CCAGG ^CC[AT]GG
130 CCTGG
MaeIII 122 GTTAC ^GT[ACGT]AC
352 GTAAC
MboII 108 GAAGA GAAGA(8/7)
316 GAAGA
MvaI 50 CCAGG CC^[AT]GG
130 CCTGG
RsaI 8 GTAC GT^AC
134 GTAC
ScrFI 50 CCAGG CC^[ACGT]GG
130 CCTGG
StuI 127 AGGCCT AGG^CCT
293 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI Cac8I CfoI Fnu4HI HaeIII
HhaI HinP1I ItaI MaeII MnlI
MwoI Tsp509I