Restriction map for
pF1KE0763
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 440 GGCGTC G[GA]^CG[CT]C
AflIII 821 ACATGT A^C[GA][CT]GT
AlwNI 166 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 617 CCTTAGG CC^T[ACGT]AGG
BalI 479 TGGCCA TGG^CCA
BbsI 292 GAAGAC GAAGAC(2/6)
BclI 359 TGATCA T^GATCA
BfaI 262 CTAG C^TAG
BpuAI 292 GAAGAC GAAGAC(2/6)
BsaAI 376 TACGTG [CT]AC^GT[GA]
BsaHI 440 GGCGTC G[GA]^CG[CT]C
BsaJI 658 CCGTGG C^C[ACGT][ACGT]GG
BsiYI 658 CCGTGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 658 CCGTGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 86 GAGACG CGTCTC(1/5)
BsmI 845 GCATTC GAATGC(1/-1)
BspLU11I 821 ACATGT A^CATGT
BssKI 506 CCAGG ^CC[ACGT]GG
BstF5I 216 CATCC GGATG(2/0)
BstNI 506 CCAGG CC^[AT]GG
Bsu36I 617 CCTTAGG CC^T[ACGT]AGG
CfoI 798 GCGC GCG^C
DsaI 658 CCGTGG C^C[GA][CT]GG
EaeI 479 TGGCCA [CT]^GGCC[GA]
EarI 930 CTCTTC CTCTTC(1/4)
Eco81I 617 CCTTAGG CC^T[ACGT]AGG
EcoRI 763 GAATTC G^AATTC
EcoRII 506 CCAGG ^CC[AT]GG
FbaI 359 TGATCA T^GATCA
FokI 216 CATCC GGATG(9/13)
HgaI 441 GCGTC GACGC(5/10)
HhaI 798 GCGC GCG^C
Hin1I 440 GGCGTC G[GA]^CG[CT]C
HinP1I 798 GCGC G^CGC
Ksp632I 930 CTCTTC CTCTTC(1/4)
MaeI 262 CTAG C^TAG
MaeIII 443 GTCAC ^GT[ACGT]AC
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MspA1I 12 CAGCGG C[AC]G^C[GT]G
MvaI 506 CCAGG CC^[AT]GG
PstI 758 CTGCAG CTGCA^G
ScrFI 506 CCAGG CC^[ACGT]GG
SfcI 758 CTGCAG C^T[GA][CT]AG
TaqI 290 TCGA T^CGA
Tsp45I 443 GTCAC ^GT[GC]AC
TthHB8I 290 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 390 GAATTT [GA]^AATT[CT]
763 GAATTC
AluI 137 AGCT AG^CT
836 AGCT
ApoI 390 GAATTT [GA]^AATT[CT]
763 GAATTC
BsmFI 279 GGGAC GGGAC(10/14)
811 GTCCC
DpnII 66 GATC ^GATC
360 GATC
DraII 279 GGGACCC [GA]G^G[ACGT]CC[CT]
856 AGGACCC
EcoO109I 279 GGGACCC [GA]G^G[ACGT]CC[CT]
856 AGGACCC
HaeIII 480 GGCC GG^CC
954 GGCC
HphI 104 TCACC GGTGA(8/7)
323 TCACC
MboI 66 GATC ^GATC
360 GATC
MslI 229 CACTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
868 CACCAATATG
NdeII 66 GATC ^GATC
360 GATC
NspI 224 ACATGC [GA]CATG^[CT]
821 ACATGT
PpuMI 279 GGGACCC [GA]G^G[AT]CC[CT]
856 AGGACCC
Sau3AI 66 GATC ^GATC
360 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AvaII BbvI BsmAI
BsrI Cac8I Cfr13I DdeI Fnu4HI
HinfI ItaI MaeII MboII MnlI
MseI MwoI NlaIII NlaIV RsaI
Sau96I SfaNI TfiI Tru9I TseI
Tsp509I TspRI