Restriction map for
pF1KB0971
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbsI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MluNI MscI MslI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 251 TCCGGA T^CCGGA
AlwNI 106 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 540 GGGCCC GGGCC^C
AvaII 331 GGACC G^G[AT]CC
AviII 146 TGCGCA TGC^GCA
BbvCI 103 CCTCAGC CCTCAGC(-5/-2)
BcgI 149 GCATGGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 251 TCCGGA T^CCGGA
BseRI 64 GAGGAG GAGGAG(10/8)
BsmAI 407 GAGAC GTCTC(1/5)
BsmI 451 GAATGC GAATGC(1/-1)
BspEI 251 TCCGGA T^CCGGA
BspMI 28 GCAGGT ACCTGC(4/8)
BsrI 205 CCAGT ACTGG(1/-1)
BstNI 334 CCTGG CC^[AT]GG
BstXI 302 CCAAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 104 CTCAG C^T[ACGT]AG
EaeI 367 CGGCCA [CT]^GGCC[GA]
EarI 52 GAAGAG CTCTTC(1/4)
EcoRII 334 CCTGG ^CC[AT]GG
FspI 146 TGCGCA TGC^GCA
HgaI 79 GACGC GACGC(5/10)
HinfI 313 GAATC G^A[ACGT]TC
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeII 143 ACGT A^CGT
MaeIII 325 GTCAC ^GT[ACGT]AC
MroI 251 TCCGGA T^CCGGA
MseI 290 TTAA T^TAA
MvaI 334 CCTGG CC^[AT]GG
NdeI 505 CATATG CA^TATG
NspI 383 GCATGC [GA]CATG^[CT]
PvuII 106 CAGCTG CAG^CTG
SexAI 333 ACCTGGT A^CC[AT]GGT
SmaI 86 CCCGGG CCC^GGG
SphI 383 GCATGC GCATG^C
TfiI 313 GAATC G^A[AT]TC
Tru9I 290 TTAA T^TAA
Tsp45I 325 GTCAC ^GT[GC]AC
XbaI 432 TCTAGA T^CTAGA
XmaCI 86 CCCGGG C^CCGGG
XmaI 86 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 57 GGCGCC G[GA]^CG[CT]C
69 GGCGCC
Alw44I 220 GTGCAC G^TGCAC
548 GTGCAC
ApaLI 220 GTGCAC G^TGCAC
548 GTGCAC
AspHI 220 GTGCAC G[AT]GC[AT]^C
548 GTGCAC
AvaI 61 CCCGAG C^[CT]CG[GA]G
86 CCCGGG
BanI 57 GGCGCC G^G[CT][GA]CC
69 GGCGCC
BanII 3 GGGCTC G[GA]GC[CT]^C
540 GGGCCC
BbeI 57 GGCGCC GGCGC^C
69 GGCGCC
BfaI 433 CTAG C^TAG
545 CTAG
BglI 40 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
85 GCCCGGGCGGC
BsaHI 57 GGCGCC G[GA]^CG[CT]C
69 GGCGCC
BsaWI 251 TCCGGA [AT]^CCGG[AT]
328 ACCGGA
BsgI 112 CTGCAC GTGCAG(16/14)
235 CTGCAC
BsiHKAI 220 GTGCAC G[AT]GC[AT]^C
548 GTGCAC
BsoBI 61 CCCGAG C^[CT]CG[GA]G
86 CCCGGG
BstF5I 318 CATCC GGATG(2/0)
210 GGATG
DpnII 398 GATC ^GATC
436 GATC
DraII 539 AGGGCCC [GA]G^G[ACGT]CC[CT]
540 GGGCCCT
EcoO109I 539 AGGGCCC [GA]G^G[ACGT]CC[CT]
540 GGGCCCT
FauI 179 CCCGC CCCGC(4/6)
121 GCGGG
FokI 318 CATCC GGATG(9/13)
210 GGATG
HaeII 57 GGCGCC [GA]GCGC^[CT]
69 GGCGCC
Hin1I 57 GGCGCC G[GA]^CG[CT]C
69 GGCGCC
KasI 57 GGCGCC G^GCGCC
69 GGCGCC
MaeI 433 CTAG C^TAG
545 CTAG
MboI 398 GATC ^GATC
436 GATC
MspA1I 106 CAGCTG C[AC]G^C[GT]G
523 CCGCTG
NarI 57 GGCGCC GG^CGCC
69 GGCGCC
NdeII 398 GATC ^GATC
436 GATC
Sau3AI 398 GATC ^GATC
436 GATC
SfaNI 125 GCATC GCATC(5/9)
497 GCATC
SfoI 57 GGCGCC GGC^GCC
69 GGCGCC
TaqI 197 TCGA T^CGA
573 TCGA
TspRI 206 CAGTG CA[GC]TG[ACGT][ACGT]^
275 CAGTG
TthHB8I 197 TCGA T^CGA
573 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstUI Cac8I CfoI Cfr13I
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboII
MnlI MspI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI StyI
TseI