Restriction map for
pF1KB9949
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AccII AccIII AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FseI FspI HaeII HincII HindII
HindIII HpaI KasI Ksp632I KspI
MaeI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 557 GACGTC GACGT^C
Acc65I 1380 GGTACC G^GTACC
AccI 1475 GTCTAC GT^[AC][GT]AC
AclI 1430 AACGTT AA^CGTT
AcyI 557 GACGTC G[GA]^CG[CT]C
AlwNI 70 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1511 GGGCCC GGGCC^C
AseI 214 ATTAAT AT^TAAT
AsnI 214 ATTAAT AT^TAAT
Asp700I 780 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1380 GGTACC G^GTACC
BbsI 1554 GAAGAC GAAGAC(2/6)
BbvCI 4 CCTCAGC CCTCAGC(-5/-2)
BciVI 159 GGATAC GTATCC(6/5)
BclI 500 TGATCA T^GATCA
BpuAI 1554 GAAGAC GAAGAC(2/6)
BsaHI 557 GACGTC G[GA]^CG[CT]C
BsaI 1082 GAGACC GGTCTC(1/5)
BsgI 1033 CTGCAC GTGCAG(16/14)
BsmAI 1082 GAGAC GTCTC(1/5)
BspMI 1031 ACCTGC ACCTGC(4/8)
BssSI 56 CTCGTG CACGAG(-5/-1)
BstXI 933 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 849 GCGC GCG^C
DraI 174 TTTAAA TTT^AAA
EaeI 1438 CGGCCA [CT]^GGCC[GA]
EcoT14I 1056 CCAAGG C^C[AT][AT]GG
EcoT22I 225 ATGCAT ATGCA^T
FauI 1066 CCCGC CCCGC(4/6)
FbaI 500 TGATCA T^GATCA
HgaI 1557 GACGC GACGC(5/10)
HhaI 849 GCGC GCG^C
Hin1I 557 GACGTC G[GA]^CG[CT]C
HinP1I 849 GCGC G^CGC
HphI 777 GGTGA GGTGA(8/7)
KpnI 1380 GGTACC GGTAC^C
NsiI 225 ATGCAT ATGCA^T
NspI 223 ACATGC [GA]CATG^[CT]
PshBI 214 ATTAAT AT^TAAT
Psp1406I 1430 AACGTT AA^CGTT
SfcI 1290 CTACAG C^T[GA][CT]AG
SmlI 549 CTCAAG C^T[CT][GA]AG
StyI 1056 CCAAGG C^C[AT][AT]GG
XcmI 1211 CCAAACGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 780 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1043 AAATTT [GA]^AATT[CT]
1156 AAATTC
AfaI 893 GTAC GT^AC
1381 GTAC
ApoI 1043 AAATTT [GA]^AATT[CT]
1156 AAATTC
BanII 986 GAGCCC G[GA]GC[CT]^C
1511 GGGCCC
BpmI 510 CTCCAG CTGGAG(16/14)
1527 CTCCAG
BsmI 1504 GAATGC GAATGC(1/-1)
449 GCATTC
BsrDI 241 CATTGC GCAATG(2/0)
1131 CATTGC
BstYI 313 GGATCT [GA]^GATC[CT]
943 GGATCT
HapII 357 CCGG C^CGG
711 CCGG
HpaII 357 CCGG C^CGG
711 CCGG
MaeII 558 ACGT A^CGT
1431 ACGT
MflI 313 GGATCT [GA]^GATC[CT]
943 GGATCT
MspI 357 CCGG C^CGG
711 CCGG
RsaI 893 GTAC GT^AC
1381 GTAC
SfaNI 208 GATGC GCATC(5/9)
545 GCATC
Tsp45I 636 GTCAC ^GT[GC]AC
1166 GTGAC
XhoII 313 GGATCT [GA]^GATC[CT]
943 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BmyI BsaJI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MfeI MnlI MseI MslI
MspA1I MunI MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I