Restriction map for
pF1KB6869
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI ClaI CpoI DpnII
DraI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HphI ItaI KasI Ksp632I KspI
MaeI MboI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TseI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 200 GGTACC G^GTACC
AciI 377 CCGC CCGC(-3/-1)
AfaI 201 GTAC GT^AC
AgeI 203 ACCGGT A^CCGGT
AocI 44 CCTTAGG CC^T[ACGT]AGG
Asp718I 200 GGTACC G^GTACC
BanI 200 GGTACC G^G[CT][GA]CC
BanII 324 GAGCTC G[GA]GC[CT]^C
BsaBI 502 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 203 ACCGGT [AT]^CCGG[AT]
BseRI 367 GAGGAG GAGGAG(10/8)
BsgI 401 CTGCAC GTGCAG(16/14)
BsmFI 40 GGGAC GGGAC(10/14)
BsrFI 203 ACCGGT [GA]^CCGG[CT]
Bsu36I 44 CCTTAGG CC^T[ACGT]AGG
Cfr10I 203 ACCGGT [GA]^CCGG[CT]
DraIII 18 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 44 CCTTAGG CC^T[ACGT]AGG
HapII 204 CCGG C^CGG
HincII 332 GTCAAC GT[CT]^[GA]AC
HindII 332 GTCAAC GT[CT]^[GA]AC
HpaII 204 CCGG C^CGG
KpnI 200 GGTACC GGTAC^C
MaeII 76 ACGT A^CGT
MaeIII 215 GTTAC ^GT[ACGT]AC
MamI 502 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 499 GAAGA GAAGA(8/7)
MslI 259 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 204 CCGG C^CGG
MwoI 173 GCTGTTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 17 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 203 ACCGGT A^CCGGT
RsaI 201 GTAC GT^AC
SacI 324 GAGCTC GAGCT^C
SfaNI 258 GCATC GCATC(5/9)
TfiI 490 GAATC G^A[AT]TC
Van91I 17 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 83 GAATTT [GA]^AATT[CT]
96 AAATTC
ApoI 83 GAATTT [GA]^AATT[CT]
96 AAATTC
AspHI 324 GAGCTC G[AT]GC[AT]^C
370 GAGCAC
AvaII 41 GGACC G^G[AT]CC
139 GGACC
BmyI 324 GAGCTC G[AGT]GC[ACT]^C
370 GAGCAC
BsiHKAI 324 GAGCTC G[AT]GC[AT]^C
370 GAGCAC
BsiYI 17 CCACTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
380 CCTGGTTGTGG
BslI 17 CCACTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
380 CCTGGTTGTGG
Bsp1286I 324 GAGCTC G[AGT]GC[ACT]^C
370 GAGCAC
BssKI 136 CCAGG ^CC[ACGT]GG
380 CCTGG
BstNI 136 CCAGG CC^[AT]GG
380 CCTGG
Cac8I 123 GCCAGC GC[ACGT]^[ACGT]GC
284 GCCTGC
Cfr13I 41 GGACC G^G[ACGT]CC
139 GGACC
DraII 40 GGGACCT [GA]G^G[ACGT]CC[CT]
138 AGGACCC
EcoO109I 40 GGGACCT [GA]G^G[ACGT]CC[CT]
138 AGGACCC
EcoRII 136 CCAGG ^CC[AT]GG
380 CCTGG
MnlI 27 GAGG CCTC(7/6)
367 GAGG
MvaI 136 CCAGG CC^[AT]GG
380 CCTGG
PleI 163 GACTC GAGTC(4/5)
353 GACTC
PpuMI 40 GGGACCT [GA]G^G[AT]CC[CT]
138 AGGACCC
Sau96I 41 GGACC G^G[ACGT]CC
139 GGACC
ScrFI 136 CCAGG CC^[ACGT]GG
380 CCTGG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BpmI BsrI DdeI HinfI
MseI NlaIV Tru9I Tsp509I TspRI