Restriction map for
pF1KB5867
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI CelII Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I FauI FbaI FseI FspI
HapII HgaI HincII HindII HpaI
HpaII KpnI KspI MfeI MluI
MroI MslI MspI MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 803 GTATAC GT^[AC][GT]AC
AccII 989 CGCG CG^CG
AcyI 1258 GGCGCC G[GA]^CG[CT]C
AfaI 955 GTAC GT^AC
AocI 841 CCTGAGG CC^T[ACGT]AGG
AspHI 921 GAGCAC G[AT]GC[AT]^C
BanI 1258 GGCGCC G^G[CT][GA]CC
BbeI 1258 GGCGCC GGCGC^C
BbrPI 1348 CACGTG CAC^GTG
BcgI 1107 CGAAGGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 660 AGATCT A^GATCT
BpmI 99 CTGGAG CTGGAG(16/14)
BsaAI 1348 CACGTG [CT]AC^GT[GA]
BsaBI 700 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1258 GGCGCC G[GA]^CG[CT]C
BsaI 743 GGTCTC GGTCTC(1/5)
BseRI 710 CTCCTC GAGGAG(10/8)
BsiHKAI 921 GAGCAC G[AT]GC[AT]^C
BsmAI 744 GTCTC GTCTC(1/5)
BsmI 1133 GCATTC GAATGC(1/-1)
BspMI 1278 ACCTGC ACCTGC(4/8)
Bst1107I 803 GTATAC GTA^TAC
BstAPI 10 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 492 GGATG GGATG(2/0)
BstUI 989 CGCG CG^CG
BstZ17I 803 GTATAC GTA^TAC
Bsu36I 841 CCTGAGG CC^T[ACGT]AGG
Eco81I 841 CCTGAGG CC^T[ACGT]AGG
EcoRI 514 GAATTC G^AATTC
EcoRV 789 GATATC GAT^ATC
EcoT22I 626 ATGCAT ATGCA^T
FokI 492 GGATG GGATG(9/13)
Hin1I 1258 GGCGCC G[GA]^CG[CT]C
HindIII 597 AAGCTT A^AGCTT
HphI 976 TCACC GGTGA(8/7)
KasI 1258 GGCGCC G^GCGCC
MaeII 1349 ACGT A^CGT
MamI 700 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 989 CGCG CG^CG
NarI 1258 GGCGCC GG^CGCC
NsiI 626 ATGCAT ATGCA^T
NspI 204 GCATGC [GA]CATG^[CT]
PmaCI 1348 CACGTG CAC^GTG
PmlI 1348 CACGTG CAC^GTG
PstI 653 CTGCAG CTGCA^G
RsaI 955 GTAC GT^AC
SfoI 1258 GGCGCC GGC^GCC
SphI 204 GCATGC GCATG^C
SspI 136 AATATT AAT^ATT
TaqI 919 TCGA T^CGA
Tsp45I 975 GTCAC ^GT[GC]AC
TthHB8I 919 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 958 CCGC CCGC(-3/-1)
1289 GCGG
AcsI 514 GAATTC [GA]^AATT[CT]
1225 AAATTT
ApoI 514 GAATTC [GA]^AATT[CT]
1225 AAATTT
BalI 231 TGGCCA TGG^CCA
1344 TGGCCA
BmyI 425 GTGCCC G[AGT]GC[ACT]^C
921 GAGCAC
Bsp1286I 425 GTGCCC G[AGT]GC[ACT]^C
921 GAGCAC
BssKI 586 CCTGG ^CC[ACGT]GG
1209 CCTGG
BstNI 586 CCTGG CC^[AT]GG
1209 CCTGG
DraI 118 TTTAAA TTT^AAA
132 TTTAAA
EcoRII 586 CCTGG ^CC[AT]GG
1209 CCTGG
EcoT14I 74 CCATGG C^C[AT][AT]GG
152 CCATGG
HaeII 185 AGCGCC [GA]GCGC^[CT]
1258 GGCGCC
MluNI 231 TGGCCA TGG^CCA
1344 TGGCCA
MscI 231 TGGCCA TGG^CCA
1344 TGGCCA
MspA1I 644 CAGCTG C[AC]G^C[GT]G
758 CAGCTG
MvaI 586 CCTGG CC^[AT]GG
1209 CCTGG
NcoI 74 CCATGG C^CATGG
152 CCATGG
PvuII 644 CAGCTG CAG^CTG
758 CAGCTG
ScrFI 586 CCTGG CC^[ACGT]GG
1209 CCTGG
StuI 451 AGGCCT AGG^CCT
943 AGGCCT
StyI 74 CCATGG C^C[AT][AT]GG
152 CCATGG
Tsp509I 515 AATT ^AATT
1226 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AlwI AvaII BbsI BbvI
BfaI BpuAI BsaJI BsiYI BslI
BsmFI BsrI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I SfcI Tru9I TseI
TspRI XhoII