Restriction map for
pF1KE4446
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BseRI
BsiWI BsmI BsoBI Bsp1407I BspEI
BspHI BsrBI BsrGI BsrI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 961 GGCGTC G[GA]^CG[CT]C
AfaI 605 GTAC GT^AC
Aor51HI 1103 AGCGCT AGC^GCT
AspHI 431 GAGCAC G[AT]GC[AT]^C
BbsI 231 GAAGAC GAAGAC(2/6)
BbvCI 667 GCTGAGG CCTCAGC(-5/-2)
BcgI 218 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1233 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 817 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC
BpuAI 231 GAAGAC GAAGAC(2/6)
BsaHI 961 GGCGTC G[GA]^CG[CT]C
BsiEI 1089 CGATCG CG[GA][CT]^CG
BsiHKAI 431 GAGCAC G[AT]GC[AT]^C
BsmBI 493 CGTCTC CGTCTC(1/5)
BspDI 565 ATCGAT AT^CGAT
BspMI 1112 ACCTGC ACCTGC(4/8)
BsrDI 870 CATTGC GCAATG(2/0)
BsrFI 754 ACCGGC [GA]^CCGG[CT]
BssSI 179 CTCGTG CACGAG(-5/-1)
BstYI 718 GGATCT [GA]^GATC[CT]
CelII 817 GCTGAGC GC^T[ACGT]AGC
Cfr10I 754 ACCGGC [GA]^CCGG[CT]
ClaI 565 ATCGAT AT^CGAT
DraII 1230 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1103 AGCGCT AGC^GCT
EcoO109I 1230 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 59 CCAAGG C^C[AT][AT]GG
EcoT22I 1123 ATGCAT ATGCA^T
FauI 644 CCCGC CCCGC(4/6)
HgaI 962 GCGTC GACGC(5/10)
Hin1I 961 GGCGTC G[GA]^CG[CT]C
MaeII 1186 ACGT A^CGT
MflI 718 GGATCT [GA]^GATC[CT]
MspA1I 645 CCGCTG C[AC]G^C[GT]G
NdeI 1062 CATATG CA^TATG
NheI 268 GCTAGC G^CTAGC
NsiI 1123 ATGCAT ATGCA^T
PflMI 1058 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1089 CGATCG CGAT^CG
RsaI 605 GTAC GT^AC
SgrAI 753 CACCGGCG C[GA]^CCGG[CT]G
StyI 59 CCAAGG C^C[AT][AT]GG
Van91I 1058 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 718 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 505 AAATTC [GA]^AATT[CT]
739 AAATTT
AflIII 1002 ACATGT A^C[GA][CT]GT
1075 ACATGT
ApoI 505 AAATTC [GA]^AATT[CT]
739 AAATTT
AseI 82 ATTAAT AT^TAAT
1375 ATTAAT
AsnI 82 ATTAAT AT^TAAT
1375 ATTAAT
AvaII 72 GGACC G^G[AT]CC
1069 GGACC
BanI 306 GGTGCC G^G[CT][GA]CC
511 GGTGCC
BanII 820 GAGCCC G[GA]GC[CT]^C
903 GGGCTC
BcnI 248 CCGGG CC^[GC]GG
823 CCCGG
BfaI 269 CTAG C^TAG
1370 CTAG
BpmI 311 CTCCAG CTGGAG(16/14)
800 CTGGAG
BsaI 616 GAGACC GGTCTC(1/5)
1026 GAGACC
BsgI 292 GTGCAG GTGCAG(16/14)
625 GTGCAG
BsiYI 768 CCCAGCGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1058 CCAGCATATGG
BslI 768 CCCAGCGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1058 CCAGCATATGG
BsmFI 250 GGGAC GGGAC(10/14)
1068 GGGAC
BspLU11I 1002 ACATGT A^CATGT
1075 ACATGT
BstAPI 1242 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1354 GCAGCCTGTGC
CfoI 1104 GCGC GCG^C
1160 GCGC
EarI 19 GAAGAG CTCTTC(1/4)
745 GAAGAG
HaeII 1103 AGCGCT [GA]GCGC^[CT]
1159 GGCGCT
HhaI 1104 GCGC GCG^C
1160 GCGC
HinP1I 1104 GCGC G^CGC
1160 GCGC
Ksp632I 19 GAAGAG CTCTTC(1/4)
745 GAAGAG
MaeI 269 CTAG C^TAG
1370 CTAG
MaeIII 184 GTCAC ^GT[ACGT]AC
1109 GTGAC
MslI 997 CACGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1126 CATGTGGGTG
NciI 248 CCGGG CC^[GC]GG
823 CCCGG
PshBI 82 ATTAAT AT^TAAT
1375 ATTAAT
TfiI 475 GATTC G^A[AT]TC
809 GAATC
Tsp45I 184 GTCAC ^GT[GC]AC
1109 GTGAC
XcmI 59 CCAAGGACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
440 CCAAAGCAAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BmyI BsaJI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I