Restriction map for
pF1KB5786
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 854 AACGTT AA^CGTT
Aor51HI 2009 AGCGCT AGC^GCT
BalI 1191 TGGCCA TGG^CCA
BamHI 1938 GGATCC G^GATCC
BciVI 1293 GTATCC GTATCC(6/5)
BclI 1596 TGATCA T^GATCA
BglII 466 AGATCT A^GATCT
BlpI 1858 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1858 GCTGAGC GC^T[ACGT]AGC
BsaI 530 GAGACC GGTCTC(1/5)
BsaWI 2111 TCCGGT [AT]^CCGG[AT]
BseRI 1199 CTCCTC GAGGAG(10/8)
BsmI 1419 GAATGC GAATGC(1/-1)
BspHI 902 TCATGA T^CATGA
BspLU11I 824 ACATGT A^CATGT
BsrBI 99 CCGCTC CCGCTC(-3/-3)
BssSI 2056 CTCGTG CACGAG(-5/-1)
BstBI 355 TTCGAA TT^CGAA
BstXI 2031 CCACGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1858 GCTGAGC GC^T[ACGT]AGC
CfoI 2010 GCGC GCG^C
DraI 327 TTTAAA TTT^AAA
DraII 10 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 2009 AGCGCT AGC^GCT
EcoO109I 10 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 812 GAATTC G^AATTC
EcoRV 2124 GATATC GAT^ATC
FauI 1956 GCGGG CCCGC(4/6)
FbaI 1596 TGATCA T^GATCA
HaeII 2009 AGCGCT [GA]GCGC^[CT]
HhaI 2010 GCGC GCG^C
HinP1I 2010 GCGC G^CGC
MluNI 1191 TGGCCA TGG^CCA
MscI 1191 TGGCCA TGG^CCA
MspA1I 1544 CAGCTG C[AC]G^C[GT]G
NspI 824 ACATGT [GA]CATG^[CT]
NspV 355 TTCGAA TT^CGAA
PpuMI 10 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 854 AACGTT AA^CGTT
PvuII 1544 CAGCTG CAG^CTG
RcaI 902 TCATGA T^CATGA
ScaI 1186 AGTACT AGT^ACT
SfuI 355 TTCGAA TT^CGAA
SmlI 535 CTCAAG C^T[CT][GA]AG
SspI 2152 AATATT AAT^ATT
XbaI 891 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 287 GTCTAC GT^[AC][GT]AC
828 GTCTAC
AccII 883 CGCG CG^CG
2046 CGCG
AcyI 71 GGCGTC G[GA]^CG[CT]C
1978 GACGCC
AlwNI 656 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1490 CAGCATCTG
AspHI 107 GAGCTC G[AT]GC[AT]^C
664 GAGCTC
AvaI 1706 CCCGGG C^[CT]CG[GA]G
1942 CCCGGG
BanII 107 GAGCTC G[GA]GC[CT]^C
664 GAGCTC
BsaHI 71 GGCGTC G[GA]^CG[CT]C
1978 GACGCC
BsiHKAI 107 GAGCTC G[AT]GC[AT]^C
664 GAGCTC
BsoBI 1706 CCCGGG C^[CT]CG[GA]G
1942 CCCGGG
BstUI 883 CGCG CG^CG
2046 CGCG
BstYI 466 AGATCT [GA]^GATC[CT]
1938 GGATCC
DraIII 1207 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1555 CACTCAGTG
DsaI 1695 CCACGG C^C[GA][CT]GG
2031 CCACGG
EaeI 1191 TGGCCA [CT]^GGCC[GA]
1698 CGGCCA
Hin1I 71 GGCGTC G[GA]^CG[CT]C
1978 GACGCC
MflI 466 AGATCT [GA]^GATC[CT]
1938 GGATCC
MslI 61 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2032 CACGGAGATG
MvnI 883 CGCG CG^CG
2046 CGCG
PstI 491 CTGCAG CTGCA^G
1319 CTGCAG
SacI 107 GAGCTC GAGCT^C
664 GAGCTC
SmaI 1706 CCCGGG CCC^GGG
1942 CCCGGG
SpeI 735 ACTAGT A^CTAGT
1927 ACTAGT
XcmI 51 CCACACGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1925 CCACTAGTTCAATGG
XhoII 466 AGATCT [GA]^GATC[CT]
1938 GGATCC
XmaCI 1706 CCCGGG C^CCGGG
1942 CCCGGG
XmaI 1706 CCCGGG C^CCGGG
1942 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI