##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BsrGI       
BsrI        BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AccI          200  GTCTAC               GT^[AC][GT]AC
AcyI           67  GACGCC               G[GA]^CG[CT]C
AfaI           73  GTAC                 GT^AC
BciVI         241  GGATAC               GTATCC(6/5)
BsaAI         244  TACGTG               [CT]AC^GT[GA]
BsaHI          67  GACGCC               G[GA]^CG[CT]C
BsaI          403  GAGACC               GGTCTC(1/5)
BsiEI         348  CGACCG               CG[GA][CT]^CG
BsmAI         403  GAGAC                GTCTC(1/5)
BsrDI          59  CATTGC               GCAATG(2/0)
BssSI         235  CTCGTG               CACGAG(-5/-1)
BstAPI         87  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII        387  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           20  CGGCCA               [CT]^GGCC[GA]
EarI          259  GAAGAG               CTCTTC(1/4)
HaeII         134  GGCGCT               [GA]GCGC^[CT]
Hin1I          67  GACGCC               G[GA]^CG[CT]C
Ksp632I       259  GAAGAG               CTCTTC(1/4)
MaeII         245  ACGT                 A^CGT
MaeIII        253  GTGAC                ^GT[ACGT]AC
MseI          442  TTAA                 T^TAA
PleI          173  GACTC                GAGTC(4/5)
RsaI           73  GTAC                 GT^AC
Tru9I         442  TTAA                 T^TAA
Tsp45I        253  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII          37  CGCG                 CG^CG
               64  CGCG                
ApaI          118  GGGCCC               GGGCC^C
              162  GGGCCC              
AvaI          166  CCCGAG               C^[CT]CG[GA]G
              484  CCCGAG              
BanII         118  GGGCCC               G[GA]GC[CT]^C
              162  GGGCCC              
BbvI          463  GCAGC                GCAGC(8/12)
               93  GCTGC               
BpmI          334  CTGGAG               CTGGAG(16/14)
              400  CTGGAG              
BsoBI         166  CCCGAG               C^[CT]CG[GA]G
              484  CCCGAG              
BsrBI           6  GAGCGG               CCGCTC(-3/-3)
              337  GAGCGG              
BstUI          37  CGCG                 CG^CG
               64  CGCG                
Cac8I         120  GCCCGC               GC[ACGT]^[ACGT]GC
              147  GCCCGC              
HgaI           67  GACGC                GACGC(5/10)
               38  GCGTC               
HphI          252  GGTGA                GGTGA(8/7)
              386  TCACC               
MspA1I        195  CCGCTG               C[AC]G^C[GT]G
              331  CCGCTG              
MvnI           37  CGCG                 CG^CG
               64  CGCG                
SfaNI         421  GATGC                GCATC(5/9)
               87  GCATC               
TfiI          301  GATTC                G^A[AT]TC
              468  GATTC               
TseI           93  GCTGC                G^C[AT]GC
              463  GCAGC               
TspRI          83  CACTG                CA[GC]TG[ACGT][ACGT]^
              366  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        BanI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       CfoI        Cfr13I      
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       TaqI        TthHB8I