Restriction map for
pF1KB7990
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AlwI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 103 AAATTC [GA]^AATT[CT]
AcyI 431 GACGCC G[GA]^CG[CT]C
AfaI 3 GTAC GT^AC
AhdI 28 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 725 AGCGCT AGC^GCT
ApoI 103 AAATTC [GA]^AATT[CT]
AspEI 28 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 841 GTCTTC GAAGAC(2/6)
BfaI 854 CTAG C^TAG
BlpI 511 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 511 GCTGAGC GC^T[ACGT]AGC
BpuAI 841 GTCTTC GAAGAC(2/6)
BsaHI 431 GACGCC G[GA]^CG[CT]C
BsiEI 595 CGATCG CG[GA][CT]^CG
BsmI 136 GCATTC GAATGC(1/-1)
BsrDI 750 CATTGC GCAATG(2/0)
BsrI 565 CCAGT ACTGG(1/-1)
BstAPI 618 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 798 GGTGACC G^GT[ACGT]ACC
BstPI 798 GGTGACC G^GT[ACGT]ACC
BstXI 962 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 511 GCTGAGC GC^T[ACGT]AGC
DraII 173 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 326 CCGTGG C^C[GA][CT]GG
EaeI 93 CGGCCA [CT]^GGCC[GA]
Eam1105I 28 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 725 AGCGCT AGC^GCT
EcoO109I 173 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 798 GGTGACC G^GT[ACGT]ACC
EcoT14I 902 CCAAGG C^C[AT][AT]GG
Hin1I 431 GACGCC G[GA]^CG[CT]C
MaeI 854 CTAG C^TAG
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 595 CGATCG CGAT^CG
PvuII 352 CAGCTG CAG^CTG
RsaI 3 GTAC GT^AC
StuI 492 AGGCCT AGG^CCT
StyI 902 CCAAGG C^C[AT][AT]GG
TaqI 832 TCGA T^CGA
TfiI 892 GAATC G^A[AT]TC
Tsp509I 104 AATT ^AATT
TthHB8I 832 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 172 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
605 CAGCCCCTG
AvaII 174 GGACC G^G[AT]CC
933 GGACC
AviII 60 TGCGCA TGC^GCA
626 TGCGCA
BanI 319 GGCACC G^G[CT][GA]CC
826 GGCACC
BanII 670 GAGCCC G[GA]GC[CT]^C
775 GAGCCC
BclI 203 TGATCA T^GATCA
305 TGATCA
BsgI 781 CTGCAC GTGCAG(16/14)
190 GTGCAG
BsmAI 463 GAGAC GTCTC(1/5)
357 GTCTC
BspMI 176 ACCTGC ACCTGC(4/8)
810 ACCTGC
DdeI 126 CTCAG C^T[ACGT]AG
512 CTGAG
EarI 364 GAAGAG CTCTTC(1/4)
679 GAAGAG
FbaI 203 TGATCA T^GATCA
305 TGATCA
FspI 60 TGCGCA TGC^GCA
626 TGCGCA
Ksp632I 364 GAAGAG CTCTTC(1/4)
679 GAAGAG
PflMI 292 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
604 CCAGCCCCTGG
SfaNI 383 GCATC GCATC(5/9)
880 GCATC
SmaI 16 CCCGGG CCC^GGG
38 CCCGGG
Van91I 292 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
604 CCAGCCCCTGG
XmaCI 16 CCCGGG C^CCGGG
38 CCCGGG
XmaI 16 CCCGGG C^CCGGG
38 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EcoNI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I TspRI