Restriction map for
pF1KE4513
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BfaI BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI KspI MaeI
MaeII MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1360 CGCG CG^CG
AccIII 953 TCCGGA T^CCGGA
AcsI 1048 GAATTT [GA]^AATT[CT]
AflII 1128 CTTAAG C^TTAAG
AgeI 1009 ACCGGT A^CCGGT
AhdI 978 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 49 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 192 GGGCCC GGGCC^C
ApoI 1048 GAATTT [GA]^AATT[CT]
AspEI 978 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1586 GGATCC G^GATCC
BciVI 956 GGATAC GTATCC(6/5)
BfrI 1128 CTTAAG C^TTAAG
BlpI 873 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 873 GCTCAGC GC^T[ACGT]AGC
BsaBI 1259 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 953 TCCGGA T^CCGGA
BsgI 497 GTGCAG GTGCAG(16/14)
Bsp1407I 228 TGTACA T^GTACA
BspEI 953 TCCGGA T^CCGGA
BsrGI 228 TGTACA T^GTACA
BssSI 412 CTCGTG CACGAG(-5/-1)
Bst1107I 395 GTATAC GTA^TAC
BstUI 1360 CGCG CG^CG
BstXI 521 CCATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 395 GTATAC GTA^TAC
CelII 873 GCTCAGC GC^T[ACGT]AGC
CfoI 969 GCGC GCG^C
DrdI 765 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 978 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HhaI 969 GCGC GCG^C
HinP1I 969 GCGC G^CGC
HpaI 717 GTTAAC GTT^AAC
MamI 1259 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 953 TCCGGA T^CCGGA
MvnI 1360 CGCG CG^CG
NaeI 1142 GCCGGC GCC^GGC
NgoMIV 1142 GCCGGC G^CCGGC
NspI 57 GCATGT [GA]CATG^[CT]
PflMI 776 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1009 ACCGGT A^CCGGT
PvuII 1278 CAGCTG CAG^CTG
SacI 886 GAGCTC GAGCT^C
SalI 630 GTCGAC G^TCGAC
SmlI 1128 CTTAAG C^T[CT][GA]AG
SspBI 228 TGTACA T^GTACA
TfiI 1488 GAATC G^A[AT]TC
Van91I 776 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 640 CTCGGG C^[CT]CG[GA]G
1450 CTCGGG
BanI 215 GGTGCC G^G[CT][GA]CC
603 GGTGCC
BcnI 611 CCCGG CC^[GC]GG
676 CCCGG
BglI 284 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1186 GCCATCTTGGC
BglII 113 AGATCT A^GATCT
911 AGATCT
BpmI 1072 CTCCAG CTGGAG(16/14)
1620 CTCCAG
BsaI 422 GAGACC GGTCTC(1/5)
1480 GAGACC
BseRI 1447 CTCCTC GAGGAG(10/8)
883 GAGGAG
BsoBI 640 CTCGGG C^[CT]CG[GA]G
1450 CTCGGG
BsrFI 1009 ACCGGT [GA]^CCGG[CT]
1142 GCCGGC
Cfr10I 1009 ACCGGT [GA]^CCGG[CT]
1142 GCCGGC
DraII 171 AGGGCCT [GA]G^G[ACGT]CC[CT]
812 AGGCCCT
DsaI 188 CCATGG C^C[GA][CT]GG
272 CCATGG
EcoNI 900 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1577 CCTCCCAGAGG
EcoO109I 171 AGGGCCT [GA]G^G[ACGT]CC[CT]
812 AGGCCCT
MseI 718 TTAA T^TAA
1129 TTAA
NciI 611 CCCGG CC^[GC]GG
676 CCCGG
NcoI 188 CCATGG C^CATGG
272 CCATGG
StuI 1574 AGGCCT AGG^CCT
1611 AGGCCT
Tru9I 718 TTAA T^TAA
1129 TTAA
Tsp45I 325 GTGAC ^GT[GC]AC
944 GTGAC
Tsp509I 1049 AATT ^AATT
1304 AATT
XcmI 776 CCATACGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1074 CCAGATCATCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbsI
BbvI BmyI BpuAI BsaJI BsaWI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TspRI TthHB8I
XhoII