Restriction map for
pF1KE2494
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 307 GTAGAC GT^[AC][GT]AC
AocI 771 CCTGAGG CC^T[ACGT]AGG
ApaI 4 GGGCCC GGGCC^C
Asp700I 156 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 754 GAGCAC G[AT]GC[AT]^C
AvaI 205 CCCGAG C^[CT]CG[GA]G
BalI 332 TGGCCA TGG^CCA
BanII 4 GGGCCC G[GA]GC[CT]^C
BcgI 408 CGAGTTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 128 TGATCA T^GATCA
BcnI 19 CCGGG CC^[GC]GG
BglII 924 AGATCT A^GATCT
BsaI 802 GAGACC GGTCTC(1/5)
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
BsoBI 205 CCCGAG C^[CT]CG[GA]G
BspMI 1079 ACCTGC ACCTGC(4/8)
BstYI 924 AGATCT [GA]^GATC[CT]
Bsu36I 771 CCTGAGG CC^T[ACGT]AGG
DraIII 832 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 60 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 332 TGGCCA [CT]^GGCC[GA]
Eco81I 771 CCTGAGG CC^T[ACGT]AGG
FbaI 128 TGATCA T^GATCA
HincII 739 GTCAAC GT[CT]^[GA]AC
HindII 739 GTCAAC GT[CT]^[GA]AC
HphI 478 GGTGA GGTGA(8/7)
MaeII 988 ACGT A^CGT
MflI 924 AGATCT [GA]^GATC[CT]
MluNI 332 TGGCCA TGG^CCA
MscI 332 TGGCCA TGG^CCA
MslI 423 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 19 CCGGG CC^[GC]GG
NspI 365 GCATGT [GA]CATG^[CT]
PflMI 831 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 490 CTGCAG CTGCA^G
SapI 691 GAAGAGC GCTCTTC(1/4)
SfcI 490 CTGCAG C^T[GA][CT]AG
SmlI 889 CTCAAG C^T[CT][GA]AG
SspI 505 AATATT AAT^ATT
TfiI 827 GAATC G^A[AT]TC
Tsp45I 700 GTGAC ^GT[GC]AC
Van91I 831 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 924 AGATCT [GA]^GATC[CT]
XmnI 156 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 27 GCGG CCGC(-3/-1)
1042 GCGG
AlwI 252 GATCC GGATC(4/5)
859 GATCC
BbsI 280 GAAGAC GAAGAC(2/6)
984 GAAGAC
BbvCI 583 GCTGAGG CCTCAGC(-5/-2)
1090 GCTGAGG
BmyI 4 GGGCCC G[AGT]GC[ACT]^C
754 GAGCAC
BpuAI 280 GAAGAC GAAGAC(2/6)
984 GAAGAC
BsaJI 206 CCGAGG C^C[ACGT][ACGT]GG
806 CCTGGG
BsmAI 802 GAGAC GTCTC(1/5)
883 GAGAC
BsmFI 58 GGGAC GGGAC(10/14)
970 GTCCC
Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C
754 GAGCAC
BsrI 977 ACTGG ACTGG(1/-1)
1106 ACTGG
EarI 691 GAAGAG CTCTTC(1/4)
727 GAAGAG
HapII 19 CCGG C^CGG
787 CCGG
HpaII 19 CCGG C^CGG
787 CCGG
Ksp632I 691 GAAGAG CTCTTC(1/4)
727 GAAGAG
MaeIII 700 GTGAC ^GT[ACGT]AC
1033 GTTAC
MspA1I 326 CAGCTG C[AC]G^C[GT]G
581 CAGCTG
MspI 19 CCGG C^CGG
787 CCGG
PpuMI 455 AGGACCC [GA]G^G[AT]CC[CT]
1076 AGGACCT
PvuII 326 CAGCTG CAG^CTG
581 CAGCTG
Tsp509I 233 AATT ^AATT
467 AATT
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
912 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BpmI BseRI BsiYI BslI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
TaqI TseI TthHB8I