Restriction map for
pF1KE4379
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BanI BbeI BbrPI BcgI
BciVI BclI BfaI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflII 349 CTTAAG C^TTAAG
AspI 1018 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 875 CCCGGG C^[CT]CG[GA]G
BamHI 800 GGATCC G^GATCC
BanII 667 GAGCCC G[GA]GC[CT]^C
BbvCI 1063 GCTGAGG CCTCAGC(-5/-2)
BfrI 349 CTTAAG C^TTAAG
BmyI 667 GAGCCC G[AGT]GC[ACT]^C
BsaBI 1045 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 878 GGGAC GGGAC(10/14)
BsoBI 875 CCCGGG C^[CT]CG[GA]G
Bsp1286I 667 GAGCCC G[AGT]GC[ACT]^C
BspMI 129 GCAGGT ACCTGC(4/8)
BsrDI 672 CATTGC GCAATG(2/0)
BsrFI 174 GCCGGT [GA]^CCGG[CT]
BstBI 472 TTCGAA TT^CGAA
BstXI 308 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 756 GCGC GCG^C
Cfr10I 174 GCCGGT [GA]^CCGG[CT]
DraI 135 TTTAAA TTT^AAA
DraII 245 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 805 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 245 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 847 GAATTC G^AATTC
HhaI 756 GCGC GCG^C
HinP1I 756 GCGC G^CGC
HincII 640 GTTGAC GT[CT]^[GA]AC
HindII 640 GTTGAC GT[CT]^[GA]AC
HindIII 301 AAGCTT A^AGCTT
MaeII 747 ACGT A^CGT
MamI 1045 GATGACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 298 GCCAAGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 80 CATATG CA^TATG
NlaIV 800 GGATCC GG[ACGT]^[ACGT]CC
NspV 472 TTCGAA TT^CGAA
PflFI 1018 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 523 GACTC GAGTC(4/5)
PpuMI 245 AGGTCCT [GA]G^G[AT]CC[CT]
SfcI 263 CTATAG C^T[GA][CT]AG
SfuI 472 TTCGAA TT^CGAA
SmaI 875 CCCGGG CCC^GGG
TfiI 1035 GATTC G^A[AT]TC
Tth111I 1018 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 875 CCCGGG C^CCGGG
XmaI 875 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 580 AAATTC [GA]^AATT[CT]
847 GAATTC
AlwNI 1010 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1020 CAGTGTCTG
ApoI 580 AAATTC [GA]^AATT[CT]
847 GAATTC
Asp700I 117 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
957 GAAGACTTTC
AvaII 93 GGACC G^G[AT]CC
246 GGTCC
BpmI 853 CTGGAG CTGGAG(16/14)
1072 CTGGAG
BsaJI 242 CCCAGG C^C[ACGT][ACGT]GG
875 CCCGGG
BseRI 703 GAGGAG GAGGAG(10/8)
1066 GAGGAG
BstNI 243 CCAGG CC^[AT]GG
852 CCTGG
Cac8I 303 GCTTGC GC[ACGT]^[ACGT]GC
752 GCCTGC
EcoNI 44 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
861 CCTGAAACAGG
EcoRII 243 CCAGG ^CC[AT]GG
852 CCTGG
HinfI 523 GACTC G^A[ACGT]TC
1035 GATTC
HphI 355 GGTGA GGTGA(8/7)
999 GGTGA
MaeIII 193 GTCAC ^GT[ACGT]AC
1006 GTCAC
MspA1I 389 CAGCTG C[AC]G^C[GT]G
554 CAGCTG
MvaI 243 CCAGG CC^[AT]GG
852 CCTGG
NlaIII 169 CATG CATG^
779 CATG
PflMI 804 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
985 CCACAGATTGG
PvuII 389 CAGCTG CAG^CTG
554 CAGCTG
SmlI 229 CTTGAG C^T[CT][GA]AG
349 CTTAAG
TaqI 473 TCGA T^CGA
917 TCGA
Tsp45I 193 GTCAC ^GT[GC]AC
1006 GTCAC
TthHB8I 473 TCGA T^CGA
917 TCGA
Van91I 804 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
985 CCACAGATTGG
XmnI 117 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
957 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BbsI BbvI BcnI
BpuAI BsiYI BslI BsrI BssKI
BstF5I BstYI Cfr13I DdeI DpnII
EarI Fnu4HI FokI HaeIII HapII
HpaII ItaI Ksp632I MboI MboII
MflI MnlI MseI MspI NciI
NdeII Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI Tsp509I TspRI XhoII