Restriction map for
pF1KB5672
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BseRI BsgI
BsiEI BsiWI BsoBI BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI Hin1I KasI KspI
MfeI MluI MluNI MscI MslI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1244 GGTACC G^GTACC
AciI 1347 GCGG CCGC(-3/-1)
AcsI 307 AAATTC [GA]^AATT[CT]
AlwNI 497 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1579 CCTGAGG CC^T[ACGT]AGG
ApoI 307 AAATTC [GA]^AATT[CT]
AseI 166 ATTAAT AT^TAAT
AsnI 166 ATTAAT AT^TAAT
Asp718I 1244 GGTACC G^GTACC
BanI 1244 GGTACC G^G[CT][GA]CC
BcgI 1304 CGAAGATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 239 TGATCA T^GATCA
BfaI 335 CTAG C^TAG
BglII 1466 AGATCT A^GATCT
BsaBI 1149 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 81 GAGACG CGTCTC(1/5)
Bsp1407I 415 TGTACA T^GTACA
BspMI 284 ACCTGC ACCTGC(4/8)
BsrGI 415 TGTACA T^GTACA
BssSI 1453 CACGAG CACGAG(-5/-1)
BstBI 1302 TTCGAA TT^CGAA
Bsu36I 1579 CCTGAGG CC^T[ACGT]AGG
CfoI 1371 GCGC GCG^C
DraI 142 TTTAAA TTT^AAA
DrdI 874 GACTCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1579 CCTGAGG CC^T[ACGT]AGG
EcoNI 1404 CCTCATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 239 TGATCA T^GATCA
HhaI 1371 GCGC GCG^C
HinP1I 1371 GCGC G^CGC
HincII 642 GTTAAC GT[CT]^[GA]AC
HindII 642 GTTAAC GT[CT]^[GA]AC
HindIII 1064 AAGCTT A^AGCTT
HpaI 642 GTTAAC GTT^AAC
HphI 1205 TCACC GGTGA(8/7)
KpnI 1244 GGTACC GGTAC^C
MaeI 335 CTAG C^TAG
MaeII 611 ACGT A^CGT
MamI 1149 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 1302 TTCGAA TT^CGAA
PshAI 984 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 166 ATTAAT AT^TAAT
PstI 286 CTGCAG CTGCA^G
SacI 216 GAGCTC GAGCT^C
SapI 42 GAAGAGC GCTCTTC(1/4)
SexAI 1247 ACCTGGT A^CC[AT]GGT
SfcI 286 CTGCAG C^T[GA][CT]AG
SfuI 1302 TTCGAA TT^CGAA
SspBI 415 TGTACA T^GTACA
StuI 1478 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 858 GATCC GGATC(4/5)
378 GGATC
ApaI 695 GGGCCC GGGCC^C
821 GGGCCC
Asp700I 1153 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1476 GAAGGCCTTC
AspHI 216 GAGCTC G[AT]GC[AT]^C
1495 GAGCAC
BbsI 1513 GAAGAC GAAGAC(2/6)
194 GTCTTC
BpuAI 1513 GAAGAC GAAGAC(2/6)
194 GTCTTC
BsiHKAI 216 GAGCTC G[AT]GC[AT]^C
1495 GAGCAC
BsmFI 505 GTCCC GGGAC(10/14)
1040 GTCCC
BsmI 684 GAATGC GAATGC(1/-1)
104 GCATTC
BstYI 857 AGATCC [GA]^GATC[CT]
1466 AGATCT
MflI 857 AGATCC [GA]^GATC[CT]
1466 AGATCT
NcoI 8 CCATGG C^CATGG
1589 CCATGG
SspI 451 AATATT AAT^ATT
1084 AATATT
TfiI 277 GATTC G^A[AT]TC
1300 GATTC
Tsp45I 964 GTCAC ^GT[GC]AC
1280 GTGAC
XhoII 857 AGATCC [GA]^GATC[CT]
1466 AGATCT
XmnI 1153 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1476 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccIII AfaI AluI BanII BbvI
BmyI BpmI BsaI BsaJI BsaWI
BseAI BsiYI BslI BsmAI Bsp1286I
BspEI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MroI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I