Restriction map for
pF1KB5326
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AclI AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BseRI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV FbaI FseI FspI HindIII
HpaI KpnI KspI MaeII MfeI
MluI MluNI MscI MunI MvnI
NciI NcoI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccI 133 GTCGAC GT^[AC][GT]AC
AccIII 15 TCCGGA T^CCGGA
AcyI 42 GGCGCC G[GA]^CG[CT]C
AfaI 760 GTAC GT^AC
AflII 667 CTTAAG C^TTAAG
AlwNI 68 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 615 AGCGCT AGC^GCT
Asp700I 475 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 123 GAGCTC G[AT]GC[AT]^C
AvaI 22 CCCGAG C^[CT]CG[GA]G
BamHI 438 GGATCC G^GATCC
BbeI 42 GGCGCC GGCGC^C
BfrI 667 CTTAAG C^TTAAG
BsaBI 171 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GGCGCC G[GA]^CG[CT]C
BsaJI 47 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 15 TCCGGA [AT]^CCGG[AT]
BseAI 15 TCCGGA T^CCGGA
BsgI 951 GTGCAG GTGCAG(16/14)
BsiEI 89 CGGTCG CG[GA][CT]^CG
BsiHKAI 123 GAGCTC G[AT]GC[AT]^C
BsiYI 67 CCAGACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 67 CCAGACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1042 GGGAC GGGAC(10/14)
BsmI 1333 GAATGC GAATGC(1/-1)
BsoBI 22 CCCGAG C^[CT]CG[GA]G
BspEI 15 TCCGGA T^CCGGA
BsrBI 25 GAGCGG CCGCTC(-3/-3)
BsrDI 938 CATTGC GCAATG(2/0)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BssKI 605 CCAGG ^CC[ACGT]GG
BstNI 605 CCAGG CC^[AT]GG
BstYI 438 GGATCC [GA]^GATC[CT]
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
DraII 1259 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 118 GAAGAG CTCTTC(1/4)
Eco47III 615 AGCGCT AGC^GCT
EcoO109I 1259 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1307 GAATTC G^AATTC
EcoRII 605 CCAGG ^CC[AT]GG
EcoT14I 47 CCTTGG C^C[AT][AT]GG
EcoT22I 857 ATGCAT ATGCA^T
FauI 942 GCGGG CCCGC(4/6)
HgaI 70 GACGC GACGC(5/10)
Hin1I 42 GGCGCC G[GA]^CG[CT]C
HincII 133 GTCGAC GT[CT]^[GA]AC
HindII 133 GTCGAC GT[CT]^[GA]AC
HphI 817 GGTGA GGTGA(8/7)
KasI 42 GGCGCC G^GCGCC
Ksp632I 118 GAAGAG CTCTTC(1/4)
MamI 171 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 438 GGATCC [GA]^GATC[CT]
MroI 15 TCCGGA T^CCGGA
MvaI 605 CCAGG CC^[AT]GG
NaeI 39 GCCGGC GCC^GGC
NarI 42 GGCGCC GG^CGCC
NdeI 844 CATATG CA^TATG
NgoMIV 39 GCCGGC G^CCGGC
NsiI 857 ATGCAT ATGCA^T
NspI 224 ACATGC [GA]CATG^[CT]
PflMI 67 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 589 CAGCTG CAG^CTG
RsaI 760 GTAC GT^AC
SacI 123 GAGCTC GAGCT^C
SalI 133 GTCGAC G^TCGAC
ScrFI 605 CCAGG CC^[ACGT]GG
SfoI 42 GGCGCC GGC^GCC
StyI 47 CCTTGG C^C[AT][AT]GG
Van91I 67 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 438 GGATCC [GA]^GATC[CT]
XmnI 475 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 439 GATCC GGATC(4/5)
438 GGATC
AvaII 18 GGACC G^G[AT]CC
1319 GGACC
BanI 9 GGCACC G^G[CT][GA]CC
42 GGCGCC
BanII 60 GGGCTC G[GA]GC[CT]^C
123 GAGCTC
BfaI 725 CTAG C^TAG
1132 CTAG
BmyI 60 GGGCTC G[AGT]GC[ACT]^C
123 GAGCTC
Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C
123 GAGCTC
CfoI 43 GCGC GCG^C
616 GCGC
HaeII 42 GGCGCC [GA]GCGC^[CT]
615 AGCGCT
HaeIII 111 GGCC GG^CC
1260 GGCC
HhaI 43 GCGC GCG^C
616 GCGC
HinP1I 43 GCGC G^CGC
616 GCGC
MaeI 725 CTAG C^TAG
1132 CTAG
MaeIII 554 GTAAC ^GT[ACGT]AC
1036 GTAAC
MspA1I 55 CCGCTG C[AC]G^C[GT]G
589 CAGCTG
PstI 688 CTGCAG CTGCA^G
826 CTGCAG
SfaNI 859 GCATC GCATC(5/9)
1006 GCATC
SmlI 486 CTTGAG C^T[CT][GA]AG
667 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BpmI BsmAI BsrI BstF5I Cac8I
Cfr13I DdeI DpnII Fnu4HI FokI
HapII HinfI HpaII ItaI MboI
MboII MnlI MseI MslI MspI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I