Restriction map for
pF1KB6626
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AspEI AspI AviII AvrII
BalI BbeI BbsI BcgI BciVI
BclI BfaI BfrI BlnI BpuAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI BsmFI BspHI BspLU11I BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CfoI Cfr10I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 317 GGTACC G^GTACC
AccII 672 CGCG CG^CG
AccIII 842 TCCGGA T^CCGGA
AlwNI 1072 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1237 ATTAAT AT^TAAT
AsnI 1237 ATTAAT AT^TAAT
Asp700I 552 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 317 GGTACC G^GTACC
AvaI 1081 CCCGAG C^[CT]CG[GA]G
BbrPI 592 CACGTG CAC^GTG
BbvCI 408 CCTCAGC CCTCAGC(-5/-2)
BglI 693 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 554 AGATCT A^GATCT
BlpI 582 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 582 GCTGAGC GC^T[ACGT]AGC
BsaWI 842 TCCGGA [AT]^CCGG[AT]
BseAI 842 TCCGGA T^CCGGA
BseRI 1084 GAGGAG GAGGAG(10/8)
BsmI 275 GAATGC GAATGC(1/-1)
BsoBI 1081 CCCGAG C^[CT]CG[GA]G
Bsp1407I 473 TGTACA T^GTACA
BspDI 82 ATCGAT AT^CGAT
BspEI 842 TCCGGA T^CCGGA
BsrBI 7 CCGCTC CCGCTC(-3/-3)
BsrGI 473 TGTACA T^GTACA
BstUI 672 CGCG CG^CG
BstXI 717 CCAGCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 582 GCTGAGC GC^T[ACGT]AGC
ClaI 82 ATCGAT AT^CGAT
DraII 1064 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1139 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1064 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 1074 GACGC GACGC(5/10)
KpnI 317 GGTACC GGTAC^C
MroI 842 TCCGGA T^CCGGA
MseI 1238 TTAA T^TAA
MslI 858 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 672 CGCG CG^CG
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
PpuMI 1064 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1237 ATTAAT AT^TAAT
SapI 663 GAAGAGC GCTCTTC(1/4)
SexAI 1094 ACCAGGT A^CC[AT]GGT
SspBI 473 TGTACA T^GTACA
StuI 467 AGGCCT AGG^CCT
TfiI 756 GAATC G^A[AT]TC
Tru9I 1238 TTAA T^TAA
Tsp509I 568 AATT ^AATT
XmnI 552 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AspHI 589 GAGCAC G[AT]GC[AT]^C
628 GTGCTC
BamHI 3 GGATCC G^GATCC
614 GGATCC
BsaAI 343 TACGTG [CT]AC^GT[GA]
592 CACGTG
BsiHKAI 589 GAGCAC G[AT]GC[AT]^C
628 GTGCTC
BsmAI 793 GAGAC GTCTC(1/5)
730 GTCTC
DsaI 378 CCACGG C^C[GA][CT]GG
697 CCACGG
EaeI 851 CGGCCA [CT]^GGCC[GA]
1217 CGGCCA
EarI 517 CTCTTC CTCTTC(1/4)
663 GAAGAG
FauI 670 CCCGC CCCGC(4/6)
1310 GCGGG
Ksp632I 517 CTCTTC CTCTTC(1/4)
663 GAAGAG
MaeII 344 ACGT A^CGT
593 ACGT
PleI 136 GACTC GAGTC(4/5)
728 GAGTC
SmlI 952 CTCAAG C^T[CT][GA]AG
1228 CTCAAG
TspRI 1058 CACTG CA[GC]TG[ACGT][ACGT]^
1143 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TthHB8I Van91I XcmI
XhoII