Restriction map for
pF1KB6238
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 382 GACGTC GACGT^C
AcsI 1351 GAATTC [GA]^AATT[CT]
Aor51HI 861 AGCGCT AGC^GCT
ApoI 1351 GAATTC [GA]^AATT[CT]
AspHI 1292 GTGCTC G[AT]GC[AT]^C
BamHI 1205 GGATCC G^GATCC
BbeI 588 GGCGCC GGCGC^C
BfaI 889 CTAG C^TAG
BglII 1016 AGATCT A^GATCT
BsaWI 1247 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1292 GTGCTC G[AT]GC[AT]^C
BsrDI 735 CATTGC GCAATG(2/0)
BstAPI 151 GCACAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 53 CCATGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1554 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 861 AGCGCT AGC^GCT
EcoNI 1476 CCTCTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1351 GAATTC G^AATTC
HincII 1429 GTCAAC GT[CT]^[GA]AC
HindII 1429 GTCAAC GT[CT]^[GA]AC
HindIII 22 AAGCTT A^AGCTT
KasI 588 GGCGCC G^GCGCC
MaeI 889 CTAG C^TAG
NarI 588 GGCGCC GG^CGCC
NcoI 74 CCATGG C^CATGG
SfoI 588 GGCGCC GGC^GCC
SmaI 368 CCCGGG CCC^GGG
StuI 686 AGGCCT AGG^CCT
Tsp509I 1352 AATT ^AATT
XbaI 888 TCTAGA T^CTAGA
XmaCI 368 CCCGGG C^CCGGG
XmaI 368 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 779 GTAC GT^AC
1815 GTAC
BanI 372 GGTGCC G^G[CT][GA]CC
588 GGCGCC
BbsI 520 GAAGAC GAAGAC(2/6)
1098 GAAGAC
BglI 709 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1339 GCCTATCGGGC
BpuAI 520 GAAGAC GAAGAC(2/6)
1098 GAAGAC
BsrBI 1201 GAGCGG CCGCTC(-3/-3)
1571 GAGCGG
BssSI 640 CACGAG CACGAG(-5/-1)
1568 CACGAG
BstYI 1016 AGATCT [GA]^GATC[CT]
1205 GGATCC
EcoT14I 74 CCATGG C^C[AT][AT]GG
1362 CCTTGG
FauI 444 CCCGC CCCGC(4/6)
1001 CCCGC
HgaI 1101 GACGC GACGC(5/10)
1336 GACGC
MaeIII 206 GTGAC ^GT[ACGT]AC
1711 GTCAC
MflI 1016 AGATCT [GA]^GATC[CT]
1205 GGATCC
MslI 54 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
171 CATGCACATG
PpuMI 1357 GGGTCCC [GA]G^G[AT]CC[CT]
1655 AGGTCCT
PstI 1186 CTGCAG CTGCA^G
1414 CTGCAG
PvuII 109 CAGCTG CAG^CTG
917 CAGCTG
RsaI 779 GTAC GT^AC
1815 GTAC
SfcI 1186 CTGCAG C^T[GA][CT]AG
1414 CTGCAG
StyI 74 CCATGG C^C[AT][AT]GG
1362 CCTTGG
TaqI 239 TCGA T^CGA
413 TCGA
Tsp45I 206 GTGAC ^GT[GC]AC
1711 GTCAC
TthHB8I 239 TCGA T^CGA
413 TCGA
XhoII 1016 AGATCT [GA]^GATC[CT]
1205 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
ApaI AvaI AvaII BanII BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI Sau3AI Sau96I
ScrFI SfaNI TfiI TseI TspRI
Van91I XcmI