Restriction map for
pF1KB6978
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AflIII 562 ACATGT A^C[GA][CT]GT
AlwNI 258 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 715 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 254 GCTGC GCAGC(8/12)
BglII 365 AGATCT A^GATCT
BsiYI 12 CCTCTCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 12 CCTCTCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 562 ACATGT A^CATGT
BsrDI 136 GCAATG GCAATG(2/0)
BsrI 516 ACTGG ACTGG(1/-1)
DdeI 216 CTTAG C^T[ACGT]AG
DraII 8 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 92 CCACGG C^C[GA][CT]GG
EcoO109I 8 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 586 CCTTGG C^C[AT][AT]GG
HphI 537 TCACC GGTGA(8/7)
PpuMI 8 AGGACCT [GA]G^G[AT]CC[CT]
PstI 255 CTGCAG CTGCA^G
SfaNI 260 GATGC GCATC(5/9)
SmlI 371 CTCAAG C^T[CT][GA]AG
StyI 586 CCTTGG C^C[AT][AT]GG
TseI 254 GCTGC G^C[AT]GC
TspRI 523 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 715 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 253 AGCT AG^CT
677 AGCT
AlwI 595 GATCC GGATC(4/5)
519 GGATC
AspHI 70 GTGCTC G[AT]GC[AT]^C
152 GAGCAC
AvaII 9 GGACC G^G[AT]CC
89 GGACC
BmyI 70 GTGCTC G[AGT]GC[ACT]^C
152 GAGCAC
BsiHKAI 70 GTGCTC G[AT]GC[AT]^C
152 GAGCAC
BsmFI 161 GTCCC GGGAC(10/14)
476 GTCCC
Bsp1286I 70 GTGCTC G[AGT]GC[ACT]^C
152 GAGCAC
BstYI 365 AGATCT [GA]^GATC[CT]
594 AGATCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
20 CGGCCA
EarI 581 CTCTTC CTCTTC(1/4)
188 GAAGAG
Fnu4HI 19 GCGGC GC^[ACGT]GC
254 GCTGC
ItaI 19 GCGGC GC^[ACGT]GC
254 GCTGC
Ksp632I 581 CTCTTC CTCTTC(1/4)
188 GAAGAG
MaeII 25 ACGT A^CGT
107 ACGT
MflI 365 AGATCT [GA]^GATC[CT]
594 AGATCC
MseI 278 TTAA T^TAA
470 TTAA
MwoI 72 GCTCAAAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
254 GCTGCAGATGC
NspI 199 GCATGT [GA]CATG^[CT]
562 ACATGT
SfcI 114 CTGTAG C^T[GA][CT]AG
255 CTGCAG
Tru9I 278 TTAA T^TAA
470 TTAA
Tsp45I 181 GTGAC ^GT[GC]AC
559 GTGAC
XhoII 365 AGATCT [GA]^GATC[CT]
594 AGATCC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AcsI ApoI BfaI BsaJI
BsmAI BstF5I Cfr13I DpnII FokI
HaeIII HinfI MaeI MaeIII MboI
MboII MnlI NdeII NlaIII Sau3AI
Sau96I TfiI Tsp509I