Restriction map for
pF1KB7865
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BseAI BsiWI BsoBI BspDI
BspEI BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MamI MluI MroI MseI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 886 GTCTAC GT^[AC][GT]AC
AccII 720 CGCG CG^CG
AcyI 946 GACGCC G[GA]^CG[CT]C
AgeI 701 ACCGGT A^CCGGT
ApaI 715 GGGCCC GGGCC^C
AspHI 51 GAGCTC G[AT]GC[AT]^C
BalI 938 TGGCCA TGG^CCA
BanI 469 GGCACC G^G[CT][GA]CC
BclI 371 TGATCA T^GATCA
BlpI 48 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC
BsaHI 946 GACGCC G[GA]^CG[CT]C
BsgI 1432 CTGCAC GTGCAG(16/14)
BsiEI 750 CGATCG CG[GA][CT]^CG
BsiHKAI 51 GAGCTC G[AT]GC[AT]^C
BsmBI 130 GAGACG CGTCTC(1/5)
Bsp1407I 503 TGTACA T^GTACA
BspHI 1301 TCATGA T^CATGA
BsrBI 497 CCGCTC CCGCTC(-3/-3)
BsrDI 1591 GCAATG GCAATG(2/0)
BsrGI 503 TGTACA T^GTACA
BstUI 720 CGCG CG^CG
BstYI 533 AGATCC [GA]^GATC[CT]
CelII 48 GCTGAGC GC^T[ACGT]AGC
DraIII 1358 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1125 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 775 GAAGAG CTCTTC(1/4)
FbaI 371 TGATCA T^GATCA
Hin1I 946 GACGCC G[GA]^CG[CT]C
HincII 629 GTCAAC GT[CT]^[GA]AC
HindII 629 GTCAAC GT[CT]^[GA]AC
Ksp632I 775 GAAGAG CTCTTC(1/4)
MfeI 1328 CAATTG C^AATTG
MflI 533 AGATCC [GA]^GATC[CT]
MluNI 938 TGGCCA TGG^CCA
MscI 938 TGGCCA TGG^CCA
MunI 1328 CAATTG C^AATTG
MvnI 720 CGCG CG^CG
NaeI 1387 GCCGGC GCC^GGC
NgoMIV 1387 GCCGGC G^CCGGC
PflMI 849 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 701 ACCGGT A^CCGGT
PpuMI 347 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 750 CGATCG CGAT^CG
RcaI 1301 TCATGA T^CATGA
SacI 51 GAGCTC GAGCT^C
SexAI 508 ACCTGGT A^CC[AT]GGT
SmlI 274 CTCAAG C^T[CT][GA]AG
SspBI 503 TGTACA T^GTACA
TaqI 419 TCGA T^CGA
TthHB8I 419 TCGA T^CGA
Van91I 849 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 65 CCAAGGAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 533 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1532 ACATGT A^C[GA][CT]GT
1634 ACATGT
AlwI 534 GATCC GGATC(4/5)
597 GGATC
AlwNI 601 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1426 CAGCAGCTG
BcnI 56 CCGGG CC^[GC]GG
1577 CCCGG
BsaWI 332 ACCGGA [AT]^CCGG[AT]
701 ACCGGT
BsmI 799 GAATGC GAATGC(1/-1)
922 GCATTC
BspLU11I 1532 ACATGT A^CATGT
1634 ACATGT
BstEII 60 GGTCACC G^GT[ACGT]ACC
1122 GGTGACC
BstPI 60 GGTCACC G^GT[ACGT]ACC
1122 GGTGACC
BstXI 561 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCATGGGCCTGG
DraII 347 AGGACCC [GA]G^G[ACGT]CC[CT]
714 GGGGCCC
EcoO109I 347 AGGACCC [GA]G^G[ACGT]CC[CT]
714 GGGGCCC
EcoO65I 60 GGTCACC G^GT[ACGT]ACC
1122 GGTGACC
MaeII 16 ACGT A^CGT
515 ACGT
MslI 471 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1629 CACCAACATG
NciI 56 CCGGG CC^[GC]GG
1577 CCCGG
Tsp509I 545 AATT ^AATT
1329 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI