Restriction map for
pF1KB6314
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 564 GTAC GT^AC
AlwI 660 GGATC GGATC(4/5)
AlwNI 658 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 787 GAGCTC G[AT]GC[AT]^C
AvaII 467 GGTCC G^G[AT]CC
BbsI 487 GAAGAC GAAGAC(2/6)
BbvCI 426 GCTGAGG CCTCAGC(-5/-2)
BciVI 651 GTATCC GTATCC(6/5)
BcnI 591 CCGGG CC^[GC]GG
BglI 108 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 32 AGATCT A^GATCT
BpuAI 487 GAAGAC GAAGAC(2/6)
BsgI 696 CTGCAC GTGCAG(16/14)
BsiHKAI 787 GAGCTC G[AT]GC[AT]^C
BsmAI 245 GTCTC GTCTC(1/5)
BspMI 611 ACCTGC ACCTGC(4/8)
Bst1107I 583 GTATAC GTA^TAC
BstF5I 299 GGATG GGATG(2/0)
BstXI 470 CCATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 583 GTATAC GTA^TAC
DraII 68 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 318 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 68 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 278 CCTTGG C^C[AT][AT]GG
FauI 719 CCCGC CCCGC(4/6)
FokI 299 GGATG GGATG(9/13)
HapII 591 CCGG C^CGG
HincII 775 GTCAAC GT[CT]^[GA]AC
HindII 775 GTCAAC GT[CT]^[GA]AC
HpaII 591 CCGG C^CGG
HphI 666 GGTGA GGTGA(8/7)
MseI 445 TTAA T^TAA
MslI 766 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 199 CCGCTG C[AC]G^C[GT]G
MspI 591 CCGG C^CGG
NciI 591 CCGGG CC^[GC]GG
PflMI 657 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 346 GAGTC GAGTC(4/5)
RsaI 564 GTAC GT^AC
SacI 787 GAGCTC GAGCT^C
SapI 309 GCTCTTC GCTCTTC(1/4)
SfcI 319 CTACAG C^T[GA][CT]AG
SmlI 462 CTCAAG C^T[CT][GA]AG
StyI 278 CCTTGG C^C[AT][AT]GG
TfiI 811 GAATC G^A[AT]TC
Tru9I 445 TTAA T^TAA
Van91I 657 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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ApaI 68 GGGCCC GGGCC^C
716 GGGCCC
BclI 545 TGATCA T^GATCA
762 TGATCA
BpmI 74 CTCCAG CTGGAG(16/14)
515 CTGGAG
BstYI 32 AGATCT [GA]^GATC[CT]
660 GGATCT
EarI 310 CTCTTC CTCTTC(1/4)
216 GAAGAG
FbaI 545 TGATCA T^GATCA
762 TGATCA
HinfI 346 GAGTC G^A[ACGT]TC
811 GAATC
Ksp632I 310 CTCTTC CTCTTC(1/4)
216 GAAGAG
MaeII 779 ACGT A^CGT
820 ACGT
MflI 32 AGATCT [GA]^GATC[CT]
660 GGATCT
SfaNI 360 GATGC GCATC(5/9)
370 GCATC
StuI 115 AGGCCT AGG^CCT
237 AGGCCT
XhoII 32 AGATCT [GA]^GATC[CT]
660 GGATCT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccI AciI AluI BanII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp509I TspRI