##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HapII       HgaI        Hin1I       HindIII     HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AflII         711  CTTAAG               C^TTAAG
AlwI           54  GGATC                GGATC(4/5)
Aor51HI       739  AGCGCT               AGC^GCT
AvaII         232  GGACC                G^G[AT]CC
BbvCI         467  CCTCAGC              CCTCAGC(-5/-2)
BfrI          711  CTTAAG               C^TTAAG
BglII         207  AGATCT               A^GATCT
BseRI          59  GAGGAG               GAGGAG(10/8)
BsmAI         707  GAGAC                GTCTC(1/5)
BstAPI       1015  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         257  CCACTATTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         207  AGATCT               [GA]^GATC[CT]
Cac8I           3  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          740  GCGC                 GCG^C
DsaI          497  CCATGG               C^C[GA][CT]GG
Eco47III      739  AGCGCT               AGC^GCT
EcoT22I       326  ATGCAT               ATGCA^T
HaeII         739  AGCGCT               [GA]GCGC^[CT]
HaeIII        290  GGCC                 GG^CC
HhaI          740  GCGC                 GCG^C
HinP1I        740  GCGC                 G^CGC
HincII       1000  GTTGAC               GT[CT]^[GA]AC
HindII       1000  GTTGAC               GT[CT]^[GA]AC
MaeIII        539  GTAAC                ^GT[ACGT]AC
MflI          207  AGATCT               [GA]^GATC[CT]
NcoI          497  CCATGG               C^CATGG
NsiI          326  ATGCAT               ATGCA^T
PflMI         930  CCATGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          508  GAGTC                GAGTC(4/5)
ScaI           31  AGTACT               AGT^ACT
Van91I        930  CCATGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          574  TCTAGA               T^CTAGA
XhoII         207  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BsrDI         639  CATTGC               GCAATG(2/0)
              792  CATTGC              
BsrI          244  ACTGG                ACTGG(1/-1)
               71  CCAGT               
Cfr13I        232  GGACC                G^G[ACGT]CC
              289  GGGCC               
EcoT14I        50  CCAAGG               C^C[AT][AT]GG
              497  CCATGG              
FauI           42  CCCGC                CCCGC(4/6)
               76  GCGGG               
MaeII         339  ACGT                 A^CGT
              492  ACGT                
PvuII         470  CAGCTG               CAG^CTG
              809  CAGCTG              
Sau96I        232  GGACC                G^G[ACGT]CC
              289  GGGCC               
SfaNI        1065  GATGC                GCATC(5/9)
              328  GCATC               
SmlI          316  CTTGAG               C^T[CT][GA]AG
              711  CTTAAG              
StyI           50  CCAAGG               C^C[AT][AT]GG
              497  CCATGG              
TspRI          72  CAGTG                CA[GC]TG[ACGT][ACGT]^
              179  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BfaI        BsaJI       BsiYI       BslI        
BssKI       BstF5I      BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HinfI       ItaI        
MaeI        MboI        MboII       MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       TaqI        TfiI        
Tru9I       TseI        Tsp509I     TthHB8I