Restriction map for
pF1KE2342
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbvCI BciVI BfaI BfrI
BlnI BsaBI BsaI BsiWI BsmBI
BspHI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CfoI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HaeII HgaI HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MseI MslI MunI
MvnI NarI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 607 GACGTC GACGT^C
AccIII 355 TCCGGA T^CCGGA
AcsI 235 GAATTT [GA]^AATT[CT]
AcyI 607 GACGTC G[GA]^CG[CT]C
AflIII 983 ACATGT A^C[GA][CT]GT
AlwI 584 GGATC GGATC(4/5)
ApaI 83 GGGCCC GGGCC^C
ApoI 235 GAATTT [GA]^AATT[CT]
AspHI 175 GAGCAC G[AT]GC[AT]^C
BanI 379 GGTGCC G^G[CT][GA]CC
BbrPI 919 CACGTG CAC^GTG
BbsI 766 GAAGAC GAAGAC(2/6)
BcgI 11 CGACTCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 458 TGATCA T^GATCA
BcnI 863 CCGGG CC^[GC]GG
BglII 633 AGATCT A^GATCT
BlpI 123 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTAAGC GC^T[ACGT]AGC
BpuAI 766 GAAGAC GAAGAC(2/6)
BsaHI 607 GACGTC G[GA]^CG[CT]C
BsaWI 355 TCCGGA [AT]^CCGG[AT]
BseAI 355 TCCGGA T^CCGGA
BsiHKAI 175 GAGCAC G[AT]GC[AT]^C
BsmAI 520 GTCTC GTCTC(1/5)
BsmFI 1213 GGGAC GGGAC(10/14)
BsmI 1124 GCATTC GAATGC(1/-1)
Bsp1407I 986 TGTACA T^GTACA
BspDI 202 ATCGAT AT^CGAT
BspEI 355 TCCGGA T^CCGGA
BspLU11I 983 ACATGT A^CATGT
BsrBI 847 GAGCGG CCGCTC(-3/-3)
BsrFI 64 GCCGGC [GA]^CCGG[CT]
BsrGI 986 TGTACA T^GTACA
BsrI 957 CCAGT ACTGG(1/-1)
BstXI 788 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 633 AGATCT [GA]^GATC[CT]
CelII 123 GCTAAGC GC^T[ACGT]AGC
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
ClaI 202 ATCGAT AT^CGAT
DraII 867 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 52 CGGCCG C^GGCCG
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoO109I 867 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 342 GATATC GAT^ATC
FbaI 458 TGATCA T^GATCA
Hin1I 607 GACGTC G[GA]^CG[CT]C
MaeIII 374 GTTAC ^GT[ACGT]AC
MflI 633 AGATCT [GA]^GATC[CT]
MroI 355 TCCGGA T^CCGGA
NaeI 64 GCCGGC GCC^GGC
NciI 863 CCGGG CC^[GC]GG
NgoMIV 64 GCCGGC G^CCGGC
NotI 51 GCGGCCGC GC^GGCCGC
PleI 12 GACTC GAGTC(4/5)
PmaCI 919 CACGTG CAC^GTG
PmlI 919 CACGTG CAC^GTG
PvuI 204 CGATCG CGAT^CG
SfiI 183 GGCCCCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 986 TGTACA T^GTACA
StuI 1034 AGGCCT AGG^CCT
Tsp509I 236 AATT ^AATT
XcmI 626 CCAAGACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 633 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 817 GTCTAC GT^[AC][GT]AC
1018 GTCTAC
AlwNI 278 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
506 CAGGCTCTG
AvaI 347 CCCGAG C^[CT]CG[GA]G
656 CTCGGG
BalI 485 TGGCCA TGG^CCA
936 TGGCCA
BglI 184 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
870 GCCTCTTGGGC
BsaAI 820 TACGTG [CT]AC^GT[GA]
919 CACGTG
BseRI 130 GAGGAG GAGGAG(10/8)
337 GAGGAG
BsgI 916 CTGCAC GTGCAG(16/14)
1070 GTGCAG
BsiEI 52 CGGCCG CG[GA][CT]^CG
204 CGATCG
BsoBI 347 CCCGAG C^[CT]CG[GA]G
656 CTCGGG
FauI 992 CCCGC CCCGC(4/6)
81 GCGGG
MluNI 485 TGGCCA TGG^CCA
936 TGGCCA
MscI 485 TGGCCA TGG^CCA
936 TGGCCA
NcoI 155 CCATGG C^CATGG
1166 CCATGG
NspI 321 GCATGT [GA]CATG^[CT]
983 ACATGT
PvuII 247 CAGCTG CAG^CTG
1091 CAGCTG
TaqI 203 TCGA T^CGA
1182 TCGA
TfiI 777 GATTC G^A[AT]TC
1080 GATTC
TthHB8I 203 TCGA T^CGA
1182 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BspMI BsrDI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI TspRI