##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmFI       BsmI        BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TfiI        TseI        TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AcsI           72  AAATTC               [GA]^AATT[CT]
ApoI           72  AAATTC               [GA]^AATT[CT]
AvaII          96  GGTCC                G^G[AT]CC
BbsI            5  GTCTTC               GAAGAC(2/6)
BfaI           23  CTAG                 C^TAG
BglI          107  GCCTGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI           5  GTCTTC               GAAGAC(2/6)
BsmAI         231  GAGAC                GTCTC(1/5)
BsmBI         231  GAGACG               CGTCTC(1/5)
Bsp1407I      212  TGTACA               T^GTACA
BsrGI         212  TGTACA               T^GTACA
BsrI           55  CCAGT                ACTGG(1/-1)
Cac8I         103  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I         96  GGTCC                G^G[ACGT]CC
DraII          95  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          228  GAAGAG               CTCTTC(1/4)
EcoO109I       95  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       185  ATGCAT               ATGCA^T
HinfI           3  GAGTC                G^A[ACGT]TC
Ksp632I       228  GAAGAG               CTCTTC(1/4)
MaeI           23  CTAG                 C^TAG
MaeIII         26  GTGAC                ^GT[ACGT]AC
MwoI          107  GCCTGGATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        120  CATG                 CATG^
NlaIV          95  GGGTCC               GG[ACGT]^[ACGT]CC
NsiI          185  ATGCAT               ATGCA^T
PleI            3  GAGTC                GAGTC(4/5)
PpuMI          95  GGGTCCT              [GA]G^G[AT]CC[CT]
Sau96I         96  GGTCC                G^G[ACGT]CC
SfaNI         184  GATGC                GCATC(5/9)
SspBI         212  TGTACA               T^GTACA
TaqI           90  TCGA                 T^CGA
Tsp45I         26  GTGAC                ^GT[GC]AC
TthHB8I        90  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI          213  GTAC                 GT^AC
              221  GTAC                
BpmI           77  CTGGAG               CTGGAG(16/14)
              133  CTGGAG              
BsaJI         131  CCCTGG               C^C[ACGT][ACGT]GG
              195  CCGAGG              
MnlI           92  GAGG                 CCTC(7/6)
              197  GAGG                
MseI           34  TTAA                 T^TAA
               70  TTAA                
RsaI          213  GTAC                 GT^AC
              221  GTAC                
Tru9I          34  TTAA                 T^TAA
               70  TTAA                
Tsp509I        68  AATT                 ^AATT
               73  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BssKI       BstF5I      BstNI       EcoRII      FokI        
MboII       MvaI        ScrFI