Restriction map for
pF1KE1084
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 370 GTATAC GT^[AC][GT]AC
AciI 119 CCGC CCGC(-3/-1)
AcsI 167 AAATTC [GA]^AATT[CT]
AlwI 450 GATCC GGATC(4/5)
AocI 615 CCTGAGG CC^T[ACGT]AGG
ApoI 167 AAATTC [GA]^AATT[CT]
Asp700I 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 28 GGTCC G^G[AT]CC
AviII 584 TGCGCA TGC^GCA
BanII 620 GGGCTC G[GA]GC[CT]^C
BmyI 620 GGGCTC G[AGT]GC[ACT]^C
BpmI 385 CTCCAG CTGGAG(16/14)
BseRI 322 GAGGAG GAGGAG(10/8)
BsgI 427 GTGCAG GTGCAG(16/14)
Bsp1286I 620 GGGCTC G[AGT]GC[ACT]^C
Bst1107I 370 GTATAC GTA^TAC
BstZ17I 370 GTATAC GTA^TAC
Bsu36I 615 CCTGAGG CC^T[ACGT]AGG
Cfr13I 28 GGTCC G^G[ACGT]CC
DpnII 450 GATC ^GATC
Eco81I 615 CCTGAGG CC^T[ACGT]AGG
EcoNI 198 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 584 TGCGCA TGC^GCA
HaeII 19 AGCGCC [GA]GCGC^[CT]
MboI 450 GATC ^GATC
MseI 593 TTAA T^TAA
NdeII 450 GATC ^GATC
SapI 622 GCTCTTC GCTCTTC(1/4)
Sau3AI 450 GATC ^GATC
Sau96I 28 GGTCC G^G[ACGT]CC
TaqI 182 TCGA T^CGA
Tru9I 593 TTAA T^TAA
TthHB8I 182 TCGA T^CGA
XbaI 480 TCTAGA T^CTAGA
XmnI 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 211 ACATGT A^C[GA][CT]GT
227 ACATGT
AlwNI 454 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
550 CAGTGGCTG
BfaI 376 CTAG C^TAG
481 CTAG
BsiYI 198 CCTGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCAGCCTGAGG
BslI 198 CCTGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
611 CCAGCCTGAGG
BsmAI 208 GAGAC GTCTC(1/5)
325 GAGAC
BspLU11I 211 ACATGT A^CATGT
227 ACATGT
BstF5I 177 CATCC GGATG(2/0)
298 GGATG
CfoI 20 GCGC GCG^C
585 GCGC
EarI 144 CTCTTC CTCTTC(1/4)
623 CTCTTC
FokI 177 CATCC GGATG(9/13)
298 GGATG
HhaI 20 GCGC GCG^C
585 GCGC
HinP1I 20 GCGC G^CGC
585 GCGC
HphI 336 GGTGA GGTGA(8/7)
140 TCACC
Ksp632I 144 CTCTTC CTCTTC(1/4)
623 CTCTTC
MaeI 376 CTAG C^TAG
481 CTAG
MaeIII 215 GTAAC ^GT[ACGT]AC
224 GTAAC
MwoI 73 GCCTTCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
525 GCTGACGAAGC
NspI 211 ACATGT [GA]CATG^[CT]
227 ACATGT
Tsp509I 156 AATT ^AATT
168 AATT
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsrI
BssKI BstNI Cac8I DdeI EcoRII
Fnu4HI HinfI ItaI MboII MnlI
MvaI NlaIII PstI RsaI ScrFI
SfaNI SfcI TfiI TseI TspRI