Restriction map for
pF1KE4526
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI ApoI AseI
AsnI Asp700I AvrII BamHI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI TfiI Tru9I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 926 GGTACC G^GTACC
AhdI 574 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 235 GGCGCGCC GG^CGCGCC
Asp718I 926 GGTACC G^GTACC
AspEI 574 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1001 TGCGCA TGC^GCA
BbrPI 1606 CACGTG CAC^GTG
BglI 1222 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiWI 959 CGTACG C^GTACG
BsmBI 435 CGTCTC CGTCTC(1/5)
BsrBI 1033 GAGCGG CCGCTC(-3/-3)
BstXI 1646 CCAACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 574 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 721 GAAGAG CTCTTC(1/4)
EcoT14I 900 CCATGG C^C[AT][AT]GG
EcoT22I 1484 ATGCAT ATGCA^T
FspI 1001 TGCGCA TGC^GCA
HinfI 1633 GAGTC G^A[ACGT]TC
KpnI 926 GGTACC GGTAC^C
Ksp632I 721 GAAGAG CTCTTC(1/4)
MaeIII 1460 GTGAC ^GT[ACGT]AC
NcoI 900 CCATGG C^CATGG
NsiI 1484 ATGCAT ATGCA^T
NspI 1486 GCATGC [GA]CATG^[CT]
PflFI 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1356 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1633 GAGTC GAGTC(4/5)
PmaCI 1606 CACGTG CAC^GTG
PmlI 1606 CACGTG CAC^GTG
PshAI 874 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 721 GAAGAGC GCTCTTC(1/4)
SphI 1486 GCATGC GCATG^C
StuI 863 AGGCCT AGG^CCT
StyI 900 CCATGG C^C[AT][AT]GG
Tsp45I 1460 GTGAC ^GT[GC]AC
Tsp509I 1710 AATT ^AATT
Tth111I 1111 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1356 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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Alw44I 67 GTGCAC G^TGCAC
1603 GTGCAC
AlwI 126 GATCC GGATC(4/5)
1559 GGATC
AocI 742 CCTCAGG CC^T[ACGT]AGG
1225 CCTGAGG
Aor51HI 200 AGCGCT AGC^GCT
1478 AGCGCT
ApaI 32 GGGCCC GGGCC^C
422 GGGCCC
ApaLI 67 GTGCAC G^TGCAC
1603 GTGCAC
AspHI 67 GTGCAC G[AT]GC[AT]^C
1603 GTGCAC
BalI 363 TGGCCA TGG^CCA
1205 TGGCCA
BbvCI 196 CCTCAGC CCTCAGC(-5/-2)
1059 CCTCAGC
BpmI 979 CTGGAG CTGGAG(16/14)
1444 CTGGAG
BsgI 1153 GTGCAG GTGCAG(16/14)
1576 GTGCAG
BsiHKAI 67 GTGCAC G[AT]GC[AT]^C
1603 GTGCAC
Bsp1407I 152 TGTACA T^GTACA
833 TGTACA
BspMI 992 ACCTGC ACCTGC(4/8)
147 GCAGGT
BsrGI 152 TGTACA T^GTACA
833 TGTACA
Bsu36I 742 CCTCAGG CC^T[ACGT]AGG
1225 CCTGAGG
DpnII 126 GATC ^GATC
1560 GATC
Eco47III 200 AGCGCT AGC^GCT
1478 AGCGCT
Eco81I 742 CCTCAGG CC^T[ACGT]AGG
1225 CCTGAGG
HgaI 279 GCGTC GACGC(5/10)
580 GCGTC
HphI 1656 GGTGA GGTGA(8/7)
1643 TCACC
KspI 620 CCGCGG CCGC^GG
1098 CCGCGG
MboI 126 GATC ^GATC
1560 GATC
MboII 721 GAAGA GAAGA(8/7)
1278 GAAGA
MluNI 363 TGGCCA TGG^CCA
1205 TGGCCA
MscI 363 TGGCCA TGG^CCA
1205 TGGCCA
MslI 408 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1146 CACTTATGTG
NdeII 126 GATC ^GATC
1560 GATC
SacII 620 CCGCGG CCGC^GG
1098 CCGCGG
Sau3AI 126 GATC ^GATC
1560 GATC
SmlI 1474 CTCAAG C^T[CT][GA]AG
1597 CTCAAG
SspBI 152 TGTACA T^GTACA
833 TGTACA
TspRI 130 CAGTG CA[GC]TG[ACGT][ACGT]^
1555 CAGTG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BsaAI
BsaHI BsaI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MaeII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIII
NlaIV PvuII RsaI Sau96I ScrFI
SfaNI SfoI SmaI TaqI TseI
TthHB8I XcmI XmaCI XmaI