Restriction map for
pF1KE0666
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeIII HincII HindII HindIII
HpaI KasI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NarI NdeI
NheI NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
TspRI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 706 GAATTT [GA]^AATT[CT]
AcyI 869 GGCGTC G[GA]^CG[CT]C
AfaI 123 GTAC GT^AC
AhdI 567 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 981 GATCC GGATC(4/5)
Aor51HI 131 AGCGCT AGC^GCT
ApoI 706 GAATTT [GA]^AATT[CT]
AseI 840 ATTAAT AT^TAAT
AsnI 840 ATTAAT AT^TAAT
AspEI 567 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 211 GAGCCC G[GA]GC[CT]^C
BglII 858 AGATCT A^GATCT
BmyI 211 GAGCCC G[AGT]GC[ACT]^C
BsaHI 869 GGCGTC G[GA]^CG[CT]C
BsgI 515 CTGCAC GTGCAG(16/14)
BsmI 550 GCATTC GAATGC(1/-1)
Bsp1286I 211 GAGCCC G[AGT]GC[ACT]^C
BspMI 765 GCAGGT ACCTGC(4/8)
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BstBI 196 TTCGAA TT^CGAA
BstXI 269 CCAGAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 858 AGATCT [GA]^GATC[CT]
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
Eam1105I 567 GACCCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 131 AGCGCT AGC^GCT
EcoT14I 593 CCATGG C^C[AT][AT]GG
EcoT22I 716 ATGCAT ATGCA^T
Hin1I 869 GGCGTC G[GA]^CG[CT]C
MflI 858 AGATCT [GA]^GATC[CT]
MslI 719 CATCTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 24 GCCGGC GCC^GGC
NcoI 593 CCATGG C^CATGG
NgoMIV 24 GCCGGC G^CCGGC
NsiI 716 ATGCAT ATGCA^T
NspV 196 TTCGAA TT^CGAA
PshBI 840 ATTAAT AT^TAAT
RsaI 123 GTAC GT^AC
SapI 192 GCTCTTC GCTCTTC(1/4)
SfcI 476 CTACAG C^T[GA][CT]AG
SfuI 196 TTCGAA TT^CGAA
StyI 593 CCATGG C^C[AT][AT]GG
XcmI 791 CCAAAGAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 858 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 27 GGCACC G^G[CT][GA]CC
307 GGCACC
BbsI 117 GAAGAC GAAGAC(2/6)
223 GTCTTC
BfaI 406 CTAG C^TAG
824 CTAG
BpuAI 117 GAAGAC GAAGAC(2/6)
223 GTCTTC
BsmFI 236 GGGAC GGGAC(10/14)
697 GGGAC
BstF5I 255 CATCC GGATG(2/0)
459 GGATG
BstNI 276 CCTGG CC^[AT]GG
694 CCTGG
DsaI 240 CCGTGG C^C[GA][CT]GG
593 CCATGG
EcoRII 276 CCTGG ^CC[AT]GG
694 CCTGG
FauI 34 CCCGC CCCGC(4/6)
40 CCCGC
FokI 255 CATCC GGATG(9/13)
459 GGATG
HaeII 3 GGCGCT [GA]GCGC^[CT]
131 AGCGCT
HgaI 887 GACGC GACGC(5/10)
870 GCGTC
HinfI 265 GAATC G^A[ACGT]TC
336 GATTC
HphI 541 GGTGA GGTGA(8/7)
1051 GGTGA
MaeI 406 CTAG C^TAG
824 CTAG
MaeII 121 ACGT A^CGT
681 ACGT
MseI 841 TTAA T^TAA
1028 TTAA
MvaI 276 CCTGG CC^[AT]GG
694 CCTGG
SmlI 103 CTCAAG C^T[CT][GA]AG
316 CTCAAG
TfiI 265 GAATC G^A[AT]TC
336 GATTC
Tru9I 841 TTAA T^TAA
1028 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BpmI BsaJI BseRI BsiYI
BslI BsrI BssKI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EarI
Fnu4HI HapII HhaI HinP1I HpaII
ItaI Ksp632I MboI MboII MnlI
MspI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TthHB8I