Restriction map for
pF1KB7953
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BsaBI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MslI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 27 GGCGTC G[GA]^CG[CT]C
AocI 367 CCTCAGG CC^T[ACGT]AGG
AvaI 55 CTCGGG C^[CT]CG[GA]G
BalI 563 TGGCCA TGG^CCA
BamHI 149 GGATCC G^GATCC
BbrPI 640 CACGTG CAC^GTG
BbsI 331 GAAGAC GAAGAC(2/6)
BglI 225 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 108 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC
BpuAI 331 GAAGAC GAAGAC(2/6)
BsaAI 640 CACGTG [CT]AC^GT[GA]
BsaHI 27 GGCGTC G[GA]^CG[CT]C
BsaWI 668 ACCGGA [AT]^CCGG[AT]
BseRI 553 GAGGAG GAGGAG(10/8)
BsmAI 282 GTCTC GTCTC(1/5)
BsoBI 55 CTCGGG C^[CT]CG[GA]G
BssSI 490 CACGAG CACGAG(-5/-1)
BstXI 314 CCACGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 149 GGATCC [GA]^GATC[CT]
Bsu36I 367 CCTCAGG CC^T[ACGT]AGG
CelII 108 GCTCAGC GC^T[ACGT]AGC
DpnII 150 GATC ^GATC
DraII 50 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 21 GACAAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 563 TGGCCA [CT]^GGCC[GA]
Eco81I 367 CCTCAGG CC^T[ACGT]AGG
EcoNI 615 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 50 GGGACCT [GA]G^G[ACGT]CC[CT]
Hin1I 27 GGCGTC G[GA]^CG[CT]C
MaeII 641 ACGT A^CGT
MboI 150 GATC ^GATC
MflI 149 GGATCC [GA]^GATC[CT]
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
NdeII 150 GATC ^GATC
PmaCI 640 CACGTG CAC^GTG
PmlI 640 CACGTG CAC^GTG
PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT]
SacI 738 GAGCTC GAGCT^C
Sau3AI 150 GATC ^GATC
SgrAI 629 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 499 CTCAAG C^T[CT][GA]AG
StuI 710 AGGCCT AGG^CCT
TaqI 656 TCGA T^CGA
TspRI 192 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 656 TCGA T^CGA
XcmI 314 CCACGGGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 149 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 354 CGCG CG^CG
627 CGCG
AlwI 150 GATCC GGATC(4/5)
149 GGATC
AspHI 637 GAGCAC G[AT]GC[AT]^C
738 GAGCTC
BanII 174 GGGCTC G[GA]GC[CT]^C
738 GAGCTC
BpmI 199 CTGGAG CTGGAG(16/14)
706 CTGGAG
BsiHKAI 637 GAGCAC G[AT]GC[AT]^C
738 GAGCTC
BsmFI 50 GGGAC GGGAC(10/14)
311 GTCCC
BspHI 569 TCATGA T^CATGA
734 TCATGA
BsrFI 6 GCCGGC [GA]^CCGG[CT]
630 GCCGGC
BstUI 354 CGCG CG^CG
627 CGCG
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
630 GCCGGC
DsaI 209 CCACGG C^C[GA][CT]GG
314 CCACGG
EarI 400 CTCTTC CTCTTC(1/4)
697 GAAGAG
FauI 477 GCGGG CCCGC(4/6)
618 GCGGG
HgaI 625 GACGC GACGC(5/10)
28 GCGTC
HphI 263 TCACC GGTGA(8/7)
545 TCACC
Ksp632I 400 CTCTTC CTCTTC(1/4)
697 GAAGAG
MaeIII 19 GTGAC ^GT[ACGT]AC
688 GTGAC
MspA1I 241 CAGCTG C[AC]G^C[GT]G
748 CAGCTG
MvnI 354 CGCG CG^CG
627 CGCG
NaeI 6 GCCGGC GCC^GGC
630 GCCGGC
NgoMIV 6 GCCGGC G^CCGGC
630 GCCGGC
PstI 244 CTGCAG CTGCA^G
277 CTGCAG
PvuII 241 CAGCTG CAG^CTG
748 CAGCTG
RcaI 569 TCATGA T^CATGA
734 TCATGA
Tsp45I 19 GTGAC ^GT[GC]AC
688 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI SfcI Tru9I TseI