Restriction map for
pF1KB5543
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 5 CGCG CG^CG
AlwNI 764 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 538 GGACC G^G[AT]CC
BbsI 430 GAAGAC GAAGAC(2/6)
BclI 269 TGATCA T^GATCA
BglII 309 AGATCT A^GATCT
BpuAI 430 GAAGAC GAAGAC(2/6)
BseRI 833 GAGGAG GAGGAG(10/8)
BsmAI 707 GAGAC GTCTC(1/5)
BsmI 111 GAATGC GAATGC(1/-1)
BspHI 609 TCATGA T^CATGA
BsrBI 174 GAGCGG CCGCTC(-3/-3)
BssSI 31 CTCGTG CACGAG(-5/-1)
BstUI 5 CGCG CG^CG
DraII 53 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 696 TGGCCG [CT]^GGCC[GA]
EcoO109I 53 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 895 GAATTC G^AATTC
FbaI 269 TGATCA T^GATCA
HincII 91 GTCAAC GT[CT]^[GA]AC
HindII 91 GTCAAC GT[CT]^[GA]AC
MvnI 5 CGCG CG^CG
PflMI 715 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 749 CTGCAG CTGCA^G
RcaI 609 TCATGA T^CATGA
SapI 457 GCTCTTC GCTCTTC(1/4)
ScaI 351 AGTACT AGT^ACT
SexAI 552 ACCTGGT A^CC[AT]GGT
SmlI 452 CTCAAG C^T[CT][GA]AG
TaqI 13 TCGA T^CGA
TfiI 875 GAATC G^A[AT]TC
TthHB8I 13 TCGA T^CGA
Van91I 715 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 682 AAATTC [GA]^AATT[CT]
895 GAATTC
AfaI 352 GTAC GT^AC
662 GTAC
AlwI 520 GATCC GGATC(4/5)
841 GATCC
ApoI 682 AAATTC [GA]^AATT[CT]
895 GAATTC
BfaI 58 CTAG C^TAG
259 CTAG
BsaJI 83 CCCTGG C^C[ACGT][ACGT]GG
158 CCCTGG
BsiYI 715 CCAATACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
721 CCTGGAGCTGG
BslI 715 CCAATACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
721 CCTGGAGCTGG
BstXI 266 CCATGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
493 CCAAATTATTGG
EarI 458 CTCTTC CTCTTC(1/4)
830 GAAGAG
HphI 778 GGTGA GGTGA(8/7)
712 TCACC
Ksp632I 458 CTCTTC CTCTTC(1/4)
830 GAAGAG
MaeI 58 CTAG C^TAG
259 CTAG
NlaIV 538 GGACCC GG[ACGT]^[ACGT]CC
730 GGAGCC
PleI 709 GACTC GAGTC(4/5)
388 GAGTC
RsaI 352 GTAC GT^AC
662 GTAC
Tsp45I 206 GTGAC ^GT[GC]AC
925 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BbvI BpmI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI XhoII