Restriction map for
pF1KB5264
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BbvI BcgI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HindIII HinfI HpaI HphI KasI
KpnI KspI MamI MluI MluNI
MscI MspA1I NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI TfiI TseI Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 897 GTCTAC GT^[AC][GT]AC
AccII 45 CGCG CG^CG
AcsI 289 AAATTC [GA]^AATT[CT]
AcyI 670 GGCGTC G[GA]^CG[CT]C
AluI 705 AGCT AG^CT
Aor51HI 7 AGCGCT AGC^GCT
ApoI 289 AAATTC [GA]^AATT[CT]
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
AvaI 138 CCCGGG C^[CT]CG[GA]G
BanI 49 GGCACC G^G[CT][GA]CC
BclI 458 TGATCA T^GATCA
BfaI 64 CTAG C^TAG
BsaHI 670 GGCGTC G[GA]^CG[CT]C
BseRI 652 GAGGAG GAGGAG(10/8)
BsgI 679 GTGCAG GTGCAG(16/14)
BsmBI 757 GAGACG CGTCTC(1/5)
BsmFI 586 GTCCC GGGAC(10/14)
BsoBI 138 CCCGGG C^[CT]CG[GA]G
BspHI 881 TCATGA T^CATGA
BspMI 851 ACCTGC ACCTGC(4/8)
BsrDI 312 CATTGC GCAATG(2/0)
BsrFI 22 GCCGGC [GA]^CCGG[CT]
BstF5I 435 GGATG GGATG(2/0)
BstUI 45 CGCG CG^CG
BstYI 535 AGATCC [GA]^GATC[CT]
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
DsaI 53 CCGTGG C^C[GA][CT]GG
Eco47III 7 AGCGCT AGC^GCT
EcoNI 645 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 458 TGATCA T^GATCA
Fnu4HI 19 GCCGC GC^[ACGT]GC
FokI 435 GGATG GGATG(9/13)
HaeII 7 AGCGCT [GA]GCGC^[CT]
HgaI 671 GCGTC GACGC(5/10)
Hin1I 670 GGCGTC G[GA]^CG[CT]C
HincII 319 GTTGAC GT[CT]^[GA]AC
HindII 319 GTTGAC GT[CT]^[GA]AC
ItaI 19 GCCGC GC^[ACGT]GC
MaeI 64 CTAG C^TAG
MaeIII 163 GTCAC ^GT[ACGT]AC
MfeI 508 CAATTG C^AATTG
MflI 535 AGATCC [GA]^GATC[CT]
MunI 508 CAATTG C^AATTG
MvnI 45 CGCG CG^CG
NaeI 22 GCCGGC GCC^GGC
NdeI 194 CATATG CA^TATG
NgoMIV 22 GCCGGC G^CCGGC
NlaIV 49 GGCACC GG[ACGT]^[ACGT]CC
NspI 575 GCATGC [GA]CATG^[CT]
PshBI 478 ATTAAT AT^TAAT
RcaI 881 TCATGA T^CATGA
SapI 87 GCTCTTC GCTCTTC(1/4)
ScaI 172 AGTACT AGT^ACT
SexAI 263 ACCAGGT A^CC[AT]GGT
SmaI 138 CCCGGG CCC^GGG
SphI 575 GCATGC GCATG^C
TaqI 838 TCGA T^CGA
Tsp45I 163 GTCAC ^GT[GC]AC
TthHB8I 838 TCGA T^CGA
XhoII 535 AGATCC [GA]^GATC[CT]
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 725 TCCGGA T^CCGGA
753 TCCGGA
AlwI 536 GATCC GGATC(4/5)
819 GATCC
BciVI 811 GGATAC GTATCC(6/5)
417 GTATCC
BsaWI 725 TCCGGA [AT]^CCGG[AT]
753 TCCGGA
BseAI 725 TCCGGA T^CCGGA
753 TCCGGA
BsmAI 301 GAGAC GTCTC(1/5)
757 GAGAC
BspEI 725 TCCGGA T^CCGGA
753 TCCGGA
BstNI 264 CCAGG CC^[AT]GG
520 CCTGG
CfoI 8 GCGC GCG^C
44 GCGC
Cfr13I 96 GGCCC G^G[ACGT]CC
136 GGCCC
DdeI 387 CTTAG C^T[ACGT]AG
638 CTCAG
EarI 88 CTCTTC CTCTTC(1/4)
202 GAAGAG
EcoRII 264 CCAGG ^CC[AT]GG
520 CCTGG
HhaI 8 GCGC GCG^C
44 GCGC
HinP1I 8 GCGC G^CGC
44 GCGC
Ksp632I 88 CTCTTC CTCTTC(1/4)
202 GAAGAG
MaeII 398 ACGT A^CGT
760 ACGT
MroI 725 TCCGGA T^CCGGA
753 TCCGGA
MslI 384 CATCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
882 CATGAATATG
MvaI 264 CCAGG CC^[AT]GG
520 CCTGG
MwoI 10 GCTCTCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
58 GCCTACCTAGC
Sau96I 96 GGCCC G^G[ACGT]CC
136 GGCCC
SfaNI 328 GCATC GCATC(5/9)
383 GCATC
SspI 475 AATATT AAT^ATT
481 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI BanII BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI Cac8I DpnII FauI HaeIII
HapII HpaII MboI MboII MnlI
MseI MspI NciI NdeII NlaIII
RsaI Sau3AI ScrFI Tru9I Tsp509I
TspRI