Restriction map for
pF1KE2439
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseAI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AfaI 255 GTAC GT^AC
AvaI 96 CCCGGG C^[CT]CG[GA]G
BglI 37 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 352 GAGACC GGTCTC(1/5)
BsaWI 203 TCCGGT [AT]^CCGG[AT]
BsiEI 59 CGGTCG CG[GA][CT]^CG
BsmAI 352 GAGAC GTCTC(1/5)
BsoBI 96 CCCGGG C^[CT]CG[GA]G
BspMI 178 ACCTGC ACCTGC(4/8)
BssHII 328 GCGCGC G^CGCGC
DsaI 56 CCGCGG C^C[GA][CT]GG
EcoNI 318 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 309 CCAAGG C^C[AT][AT]GG
FauI 182 GCGGG CCCGC(4/6)
HaeII 45 GGCGCT [GA]GCGC^[CT]
HgaI 362 GCGTC GACGC(5/10)
KspI 56 CCGCGG CCGC^GG
MaeIII 364 GTCAC ^GT[ACGT]AC
MspA1I 56 CCGCGG C[AC]G^C[GT]G
RsaI 255 GTAC GT^AC
SacII 56 CCGCGG CCGC^GG
SfaNI 200 GCATC GCATC(5/9)
SmaI 96 CCCGGG CCC^GGG
StyI 309 CCAAGG C^C[AT][AT]GG
Tsp45I 364 GTCAC ^GT[GC]AC
TspRI 343 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 96 CCCGGG C^CCGGG
XmaI 96 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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ApaI 127 GGGCCC GGGCC^C
185 GGGCCC
BanII 127 GGGCCC G[GA]GC[CT]^C
185 GGGCCC
BbvI 3 GCAGC GCAGC(8/12)
143 GCAGC
BcnI 96 CCCGG CC^[GC]GG
97 CCGGG
BsrI 224 ACTGG ACTGG(1/-1)
269 ACTGG
BstAPI 146 GCAAGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
200 GCATCCGGTGC
BstF5I 201 CATCC GGATG(2/0)
357 CATCC
BstNI 122 CCTGG CC^[AT]GG
348 CCAGG
Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC
328 GCGCGC
DdeI 113 CTGAG C^T[ACGT]AG
341 CTCAG
EcoRII 122 CCTGG ^CC[AT]GG
348 CCAGG
FokI 201 CATCC GGATG(9/13)
357 CATCC
HapII 97 CCGG C^CGG
204 CCGG
HpaII 97 CCGG C^CGG
204 CCGG
MspI 97 CCGG C^CGG
204 CCGG
MvaI 122 CCTGG CC^[AT]GG
348 CCAGG
NciI 96 CCCGG CC^[GC]GG
97 CCGGG
TseI 3 GCAGC G^C[AT]GC
143 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccII AciI BanI BmyI BsaJI
BseRI BsgI BsiYI BslI Bsp1286I
BssKI BstUI CfoI Cfr13I DraII
EcoO109I Fnu4HI HaeIII HhaI HinP1I
HphI ItaI MboII MnlI MvnI
MwoI NlaIV Sau96I ScrFI