Restriction map for
pF1KB7688
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DsaI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 505 GTCTAC GT^[AC][GT]AC
AccII 502 CGCG CG^CG
AcsI 838 AAATTT [GA]^AATT[CT]
AhdI 497 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 838 AAATTT [GA]^AATT[CT]
Asp700I 673 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 497 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 420 CGAGCTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 997 TACGTG [CT]AC^GT[GA]
BsaWI 1164 TCCGGT [AT]^CCGG[AT]
BsrBI 121 CCGCTC CCGCTC(-3/-3)
BsrDI 171 CATTGC GCAATG(2/0)
BsrI 1082 CCAGT ACTGG(1/-1)
BssSI 91 CTCGTG CACGAG(-5/-1)
BstUI 502 CGCG CG^CG
DpnII 564 GATC ^GATC
DraII 341 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1161 GACTCCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 497 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 341 AGGGCCT [GA]G^G[ACGT]CC[CT]
MboI 564 GATC ^GATC
MspA1I 1143 CAGCGG C[AC]G^C[GT]G
MvnI 502 CGCG CG^CG
NaeI 743 GCCGGC GCC^GGC
NdeII 564 GATC ^GATC
NgoMIV 743 GCCGGC G^CCGGC
NspI 723 GCATGC [GA]CATG^[CT]
PflMI 177 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 819 CTGCAG CTGCA^G
Sau3AI 564 GATC ^GATC
SexAI 704 ACCTGGT A^CC[AT]GGT
SmaI 70 CCCGGG CCC^GGG
SmlI 40 CTCAAG C^T[CT][GA]AG
SphI 723 GCATGC GCATG^C
TaqI 815 TCGA T^CGA
TthHB8I 815 TCGA T^CGA
Van91I 177 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 70 CCCGGG C^CCGGG
XmaI 70 CCCGGG C^CCGGG
XmnI 673 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 194 GTAC GT^AC
1140 GTAC
AvaI 70 CCCGGG C^[CT]CG[GA]G
124 CTCGGG
AvaII 392 GGACC G^G[AT]CC
975 GGTCC
BsaI 204 GAGACC GGTCTC(1/5)
1133 GGTCTC
BsoBI 70 CCCGGG C^[CT]CG[GA]G
124 CTCGGG
BspMI 773 ACCTGC ACCTGC(4/8)
1048 GCAGGT
BstXI 965 CCAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
980 CCAAGGCTCTGG
CfoI 312 GCGC GCG^C
501 GCGC
FauI 120 CCCGC CCCGC(4/6)
920 GCGGG
HgaI 214 GCGTC GACGC(5/10)
503 GCGTC
HhaI 312 GCGC GCG^C
501 GCGC
HinP1I 312 GCGC G^CGC
501 GCGC
HphI 102 TCACC GGTGA(8/7)
292 TCACC
MaeIII 1000 GTGAC ^GT[ACGT]AC
1085 GTGAC
MslI 222 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CATAGAAGTG
RsaI 194 GTAC GT^AC
1140 GTAC
SfcI 682 CTACAG C^T[GA][CT]AG
819 CTGCAG
Tsp45I 1000 GTGAC ^GT[GC]AC
1085 GTGAC
TspRI 438 CAGTG CA[GC]TG[ACGT][ACGT]^
1083 CAGTG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI BalI BbsI BbvI
BcnI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsrFI BssKI BstAPI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
ItaI MaeII MboII MluNI MnlI
MscI MseI MspI MvaI MwoI
NciI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp509I