Restriction map for
pF1KB3464
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MspI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 525 GTCTAC GT^[AC][GT]AC
AlwNI 760 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 313 CCTCAGG CC^T[ACGT]AGG
Asp700I 290 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 466 GGCACC G^G[CT][GA]CC
BanII 695 GGGCTC G[GA]GC[CT]^C
BbsI 802 GTCTTC GAAGAC(2/6)
BfaI 859 CTAG C^TAG
BglI 854 GCCATCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 607 CTCCAG CTGGAG(16/14)
BpuAI 802 GTCTTC GAAGAC(2/6)
BsgI 601 GTGCAG GTGCAG(16/14)
BspMI 755 ACCTGC ACCTGC(4/8)
BsrDI 774 CATTGC GCAATG(2/0)
BsrI 68 CCAGT ACTGG(1/-1)
Bsu36I 313 CCTCAGG CC^T[ACGT]AGG
Cac8I 649 GCCCGC GC[ACGT]^[ACGT]GC
CfoI 653 GCGC GCG^C
DsaI 713 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eco81I 313 CCTCAGG CC^T[ACGT]AGG
EcoNI 324 CCTCACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 686 ATGCAT ATGCA^T
HgaI 637 GCGTC GACGC(5/10)
HhaI 653 GCGC GCG^C
HinP1I 653 GCGC G^CGC
MaeI 859 CTAG C^TAG
MaeIII 340 GTGAC ^GT[ACGT]AC
MfeI 797 CAATTG C^AATTG
MseI 581 TTAA T^TAA
MspA1I 37 CAGCGG C[AC]G^C[GT]G
MunI 797 CAATTG C^AATTG
NcoI 713 CCATGG C^CATGG
NsiI 686 ATGCAT ATGCA^T
NspI 518 ACATGC [GA]CATG^[CT]
PpuMI 231 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 234 ACCTGGT A^CC[AT]GGT
Tru9I 581 TTAA T^TAA
Tsp45I 340 GTGAC ^GT[GC]AC
XcmI 139 CCAATCTCAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 290 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 321 GATCC GGATC(4/5)
486 GATCC
AvaII 82 GGACC G^G[AT]CC
232 GGACC
BmyI 465 GGGCAC G[AGT]GC[ACT]^C
695 GGGCTC
BseRI 311 CTCCTC GAGGAG(10/8)
221 GAGGAG
BsmFI 23 GGGAC GGGAC(10/14)
639 GTCCC
BsmI 381 GAATGC GAATGC(1/-1)
684 GAATGC
Bsp1286I 465 GGGCAC G[AGT]GC[ACT]^C
695 GGGCTC
BstYI 320 AGATCC [GA]^GATC[CT]
485 AGATCC
DpnII 321 GATC ^GATC
486 GATC
DraII 74 AGGGCCT [GA]G^G[ACGT]CC[CT]
231 AGGACCT
EcoO109I 74 AGGGCCT [GA]G^G[ACGT]CC[CT]
231 AGGACCT
FauI 641 CCCGC CCCGC(4/6)
650 CCCGC
HphI 122 TCACC GGTGA(8/7)
407 TCACC
MboI 321 GATC ^GATC
486 GATC
MboII 502 GAAGA GAAGA(8/7)
803 TCTTC
MflI 320 AGATCC [GA]^GATC[CT]
485 AGATCC
MslI 273 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
816 CATAGATATG
NdeII 321 GATC ^GATC
486 GATC
Sau3AI 321 GATC ^GATC
486 GATC
XhoII 320 AGATCC [GA]^GATC[CT]
485 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstUI Cfr13I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MnlI MvaI MvnI MwoI NlaIII
NlaIV PleI PstI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp509I TspRI