##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Asp700I       285  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         157  GGATCC               G^GATCC
BbvI          327  GCTGC                GCAGC(8/12)
BciVI         320  GTATCC               GTATCC(6/5)
BclI          621  TGATCA               T^GATCA
BsaJI          41  CCTTGG               C^C[ACGT][ACGT]GG
BsgI          434  GTGCAG               GTGCAG(16/14)
BsmI          392  GAATGC               GAATGC(1/-1)
BsrI          589  ACTGG                ACTGG(1/-1)
BssKI         202  CCTGG                ^CC[ACGT]GG
BstEII        648  GGTGACC              G^GT[ACGT]ACC
BstNI         202  CCTGG                CC^[AT]GG
BstPI         648  GGTGACC              G^GT[ACGT]ACC
BstXI         262  CCAGAGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         157  GGATCC               [GA]^GATC[CT]
EcoO65I       648  GGTGACC              G^GT[ACGT]ACC
EcoRII        202  CCTGG                ^CC[AT]GG
EcoT14I        41  CCTTGG               C^C[AT][AT]GG
FbaI          621  TGATCA               T^GATCA
Fnu4HI        327  GCTGC                GC^[ACGT]GC
HphI          648  GGTGA                GGTGA(8/7)
ItaI          327  GCTGC                GC^[ACGT]GC
MaeIII        649  GTGAC                ^GT[ACGT]AC
MflI          157  GGATCC               [GA]^GATC[CT]
MslI          791  CATTTACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        325  CAGCTG               C[AC]G^C[GT]G
MvaI          202  CCTGG                CC^[AT]GG
PvuII         325  CAGCTG               CAG^CTG
ScrFI         202  CCTGG                CC^[ACGT]GG
SspI          370  AATATT               AAT^ATT
StyI           41  CCTTGG               C^C[AT][AT]GG
TaqI          807  TCGA                 T^CGA
TseI          327  GCTGC                G^C[AT]GC
Tsp45I        649  GTGAC                ^GT[GC]AC
TthHB8I       807  TCGA                 T^CGA
XhoII         157  GGATCC               [GA]^GATC[CT]
XmnI          285  GAAGTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AcsI          245  AAATTC               [GA]^AATT[CT]
              809  GAATTT              
AluI          326  AGCT                 AG^CT
              549  AGCT                
ApoI          245  AAATTC               [GA]^AATT[CT]
              809  GAATTT              
BsmAI         265  GAGAC                GTCTC(1/5)
              571  GAGAC               
BstF5I         38  CATCC                GGATG(2/0)
              562  CATCC               
DdeI           74  CTGAG                C^T[ACGT]AG
              827  CTCAG               
DraI           97  TTTAAA               TTT^AAA
              711  TTTAAA              
FokI           38  CATCC                GGATG(9/13)
              562  CATCC               
MwoI           37  GCATCCTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              550  GCTGAATATGC         


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AlwI        BspHI       DpnII       HinfI       MboI        
MboII       MnlI        MseI        NdeII       NlaIII      
NlaIV       PleI        RcaI        Sau3AI      SfaNI       
TfiI        Tru9I       Tsp509I