Restriction map for
pF1KB6694
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoT22I FauI FbaI
FseI FspI HgaI Hin1I KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MslI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 757 GTCTAC GT^[AC][GT]AC
AflIII 42 ACACGT A^C[GA][CT]GT
Asp700I 901 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 398 CCCGAG C^[CT]CG[GA]G
BalI 72 TGGCCA TGG^CCA
BanII 153 GGGCTC G[GA]GC[CT]^C
BcnI 820 CCCGG CC^[GC]GG
BglI 409 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 153 GGGCTC G[AGT]GC[ACT]^C
BsaAI 43 CACGTA [CT]AC^GT[GA]
BsaI 241 GAGACC GGTCTC(1/5)
BsoBI 398 CCCGAG C^[CT]CG[GA]G
Bsp1286I 153 GGGCTC G[AGT]GC[ACT]^C
BsrFI 1063 GCCGGC [GA]^CCGG[CT]
Cfr10I 1063 GCCGGC [GA]^CCGG[CT]
Cfr13I 1168 GGCCC G^G[ACGT]CC
DraIII 425 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 328 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 220 GATATC GAT^ATC
EcoT14I 625 CCTTGG C^C[AT][AT]GG
HaeII 1060 AGCGCC [GA]GCGC^[CT]
HindIII 890 AAGCTT A^AGCTT
HpaI 454 GTTAAC GTT^AAC
MluNI 72 TGGCCA TGG^CCA
MscI 72 TGGCCA TGG^CCA
MspA1I 1010 CAGCTG C[AC]G^C[GT]G
NaeI 1063 GCCGGC GCC^GGC
NciI 820 CCCGG CC^[GC]GG
NgoMIV 1063 GCCGGC G^CCGGC
PflMI 574 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 15 GACTC GAGTC(4/5)
PvuII 1010 CAGCTG CAG^CTG
SapI 904 GCTCTTC GCTCTTC(1/4)
Sau96I 1168 GGCCC G^G[ACGT]CC
ScaI 251 AGTACT AGT^ACT
SfcI 483 CTACAG C^T[GA][CT]AG
SmlI 685 CTTGAG C^T[CT][GA]AG
StyI 625 CCTTGG C^C[AT][AT]GG
Van91I 574 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 901 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 92 GCGG CCGC(-3/-1)
1300 GCGG
AclI 850 AACGTT AA^CGTT
970 AACGTT
AfaI 252 GTAC GT^AC
779 GTAC
BlpI 193 GCTGAGC GC^T[ACGT]AGC
1117 GCTGAGC
Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC
1117 GCTGAGC
BsmFI 313 GGGAC GGGAC(10/14)
914 GGGAC
BsrDI 1101 CATTGC GCAATG(2/0)
662 GCAATG
BstEII 831 GGTCACC G^GT[ACGT]ACC
1350 GGTCACC
BstPI 831 GGTCACC G^GT[ACGT]ACC
1350 GGTCACC
CelII 193 GCTGAGC GC^T[ACGT]AGC
1117 GCTGAGC
CfoI 738 GCGC GCG^C
1061 GCGC
DpnII 81 GATC ^GATC
1138 GATC
EaeI 72 TGGCCA [CT]^GGCC[GA]
792 TGGCCG
EarI 905 CTCTTC CTCTTC(1/4)
492 GAAGAG
EcoO65I 831 GGTCACC G^GT[ACGT]ACC
1350 GGTCACC
HapII 821 CCGG C^CGG
1064 CCGG
HhaI 738 GCGC GCG^C
1061 GCGC
HinP1I 738 GCGC G^CGC
1061 GCGC
HincII 454 GTTAAC GT[CT]^[GA]AC
847 GTCAAC
HindII 454 GTTAAC GT[CT]^[GA]AC
847 GTCAAC
HpaII 821 CCGG C^CGG
1064 CCGG
Ksp632I 905 CTCTTC CTCTTC(1/4)
492 GAAGAG
MboI 81 GATC ^GATC
1138 GATC
MspI 821 CCGG C^CGG
1064 CCGG
NdeII 81 GATC ^GATC
1138 GATC
Psp1406I 850 AACGTT AA^CGTT
970 AACGTT
RsaI 252 GTAC GT^AC
779 GTAC
Sau3AI 81 GATC ^GATC
1138 GATC
SphI 405 GCATGC GCATG^C
1105 GCATGC
TaqI 101 TCGA T^CGA
875 TCGA
TfiI 49 GATTC G^A[AT]TC
466 GATTC
TthHB8I 101 TCGA T^CGA
875 TCGA
XcmI 574 CCAATTTCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1199 CCATTGTTGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI ApoI BbvI BpmI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeII MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV NspI ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI