##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AclI          137  AACGTT               AA^CGTT
AcsI          167  GAATTC               [GA]^AATT[CT]
ApoI          167  GAATTC               [GA]^AATT[CT]
BamHI         150  GGATCC               G^GATCC
BbsI          175  GAAGAC               GAAGAC(2/6)
BfaI          157  CTAG                 C^TAG
BpmI          143  CTGGAG               CTGGAG(16/14)
BpuAI         175  GAAGAC               GAAGAC(2/6)
BsmAI          87  GAGAC                GTCTC(1/5)
BstYI         150  GGATCC               [GA]^GATC[CT]
Cac8I           3  GCCTGC               GC[ACGT]^[ACGT]GC
DpnII         151  GATC                 ^GATC
DraI          190  TTTAAA               TTT^AAA
EcoRI         167  GAATTC               G^AATTC
MaeI          157  CTAG                 C^TAG
MaeII         138  ACGT                 A^CGT
MboI          151  GATC                 ^GATC
MflI          150  GGATCC               [GA]^GATC[CT]
MseI          191  TTAA                 T^TAA
MspA1I        204  CAGCTG               C[AC]G^C[GT]G
NdeII         151  GATC                 ^GATC
NlaIV         150  GGATCC               GG[ACGT]^[ACGT]CC
Psp1406I      137  AACGTT               AA^CGTT
PvuII         204  CAGCTG               CAG^CTG
Sau3AI        151  GATC                 ^GATC
TaqI           17  TCGA                 T^CGA
Tru9I         191  TTAA                 T^TAA
Tsp509I       168  AATT                 ^AATT
TthHB8I        17  TCGA                 T^CGA
XhoII         150  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           62  AGCT                 AG^CT
              205  AGCT                
AlwI          151  GATCC                GGATC(4/5)
              150  GGATC               
BbvI           60  GCAGC                GCAGC(8/12)
              203  GCAGC               
Fnu4HI         60  GCAGC                GC^[ACGT]GC
              203  GCAGC               
ItaI           60  GCAGC                GC^[ACGT]GC
              203  GCAGC               
MnlI          154  CCTC                 CCTC(7/6)
              148  GAGG                
TseI           60  GCAGC                G^C[AT]GC
              203  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII