Restriction map for
pF1KB6844
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HindIII HinfI HpaI Ksp632I
KspI MaeI MaeIII MamI MfeI
MflI MluI MroI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 226 GACGTC GACGT^C
Acc65I 290 GGTACC G^GTACC
Alw44I 564 GTGCAC G^TGCAC
AlwI 516 GATCC GGATC(4/5)
AlwNI 421 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 564 GTGCAC G^TGCAC
Asp718I 290 GGTACC G^GTACC
AvaI 437 CCCGGG C^[CT]CG[GA]G
AvaII 351 GGACC G^G[AT]CC
BalI 428 TGGCCA TGG^CCA
BanII 255 GAGCCC G[GA]GC[CT]^C
BbeI 34 GGCGCC GGCGC^C
BclI 59 TGATCA T^GATCA
BlpI 470 GCTCAGC GC^T[ACGT]AGC
BpmI 463 CTGGAG CTGGAG(16/14)
Bpu1102I 470 GCTCAGC GC^T[ACGT]AGC
BsiEI 192 CGATCG CG[GA][CT]^CG
BsoBI 437 CCCGGG C^[CT]CG[GA]G
BsrBI 379 GAGCGG CCGCTC(-3/-3)
CelII 470 GCTCAGC GC^T[ACGT]AGC
DdeI 471 CTCAG C^T[ACGT]AG
EcoT14I 431 CCATGG C^C[AT][AT]GG
FbaI 59 TGATCA T^GATCA
HaeII 34 GGCGCC [GA]GCGC^[CT]
HincII 151 GTCAAC GT[CT]^[GA]AC
HindII 151 GTCAAC GT[CT]^[GA]AC
KasI 34 GGCGCC G^GCGCC
KpnI 290 GGTACC GGTAC^C
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
NarI 34 GGCGCC GG^CGCC
NcoI 431 CCATGG C^CATGG
NspI 49 ACATGC [GA]CATG^[CT]
PvuI 192 CGATCG CGAT^CG
SfoI 34 GGCGCC GGC^GCC
SmaI 437 CCCGGG CCC^GGG
StyI 431 CCATGG C^C[AT][AT]GG
TaqI 269 TCGA T^CGA
TthHB8I 269 TCGA T^CGA
XmaCI 437 CCCGGG C^CCGGG
XmaI 437 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 34 GGCGCC G[GA]^CG[CT]C
226 GACGTC
AfaI 93 GTAC GT^AC
291 GTAC
BbvI 198 GCTGC GCAGC(8/12)
384 GCTGC
BsaHI 34 GGCGCC G[GA]^CG[CT]C
226 GACGTC
BsmFI 167 GGGAC GGGAC(10/14)
389 GGGAC
BsrI 148 CCAGT ACTGG(1/-1)
541 CCAGT
BstF5I 96 CATCC GGATG(2/0)
327 CATCC
BstXI 125 CCAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
326 CCATCCTCCTGG
CfoI 35 GCGC GCG^C
521 GCGC
DraII 422 AGGGCCT [GA]G^G[ACGT]CC[CT]
488 GGGGCCT
DsaI 38 CCGTGG C^C[GA][CT]GG
431 CCATGG
EaeI 202 CGGCCA [CT]^GGCC[GA]
428 TGGCCA
EcoO109I 422 AGGGCCT [GA]G^G[ACGT]CC[CT]
488 GGGGCCT
FokI 96 CATCC GGATG(9/13)
327 CATCC
HhaI 35 GCGC GCG^C
521 GCGC
Hin1I 34 GGCGCC G[GA]^CG[CT]C
226 GACGTC
HinP1I 35 GCGC G^CGC
521 GCGC
HphI 252 GGTGA GGTGA(8/7)
410 TCACC
MaeII 128 ACGT A^CGT
227 ACGT
MspA1I 173 CAGCGG C[AC]G^C[GT]G
484 CAGCGG
MwoI 514 GCGATCCGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
528 GCTCTGCCCGC
NlaIII 50 CATG CATG^
432 CATG
RsaI 93 GTAC GT^AC
291 GTAC
TseI 198 GCTGC G^C[AT]GC
384 GCTGC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AspHI BanI
BbsI BcnI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I Cfr13I DpnII
EcoRII FauI Fnu4HI HaeIII HapII
HpaII ItaI MboI MboII MnlI
MspI MvaI MvnI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TspRI
XcmI