Restriction map for
pF1KE1444
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# NO CUT ENZYMES ( 163 enzymes) #
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Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 45 GACGTC GACGT^C
AccIII 413 TCCGGA T^CCGGA
AspHI 14 GAGCAC G[AT]GC[AT]^C
BalI 508 TGGCCA TGG^CCA
BamHI 479 GGATCC G^GATCC
BanII 243 GAGCCC G[GA]GC[CT]^C
BciVI 201 GTATCC GTATCC(6/5)
BfaI 815 CTAG C^TAG
BsaJI 483 CCACGG C^C[ACGT][ACGT]GG
BsaWI 413 TCCGGA [AT]^CCGG[AT]
BseAI 413 TCCGGA T^CCGGA
BseRI 736 GAGGAG GAGGAG(10/8)
BsiHKAI 14 GAGCAC G[AT]GC[AT]^C
BsmAI 652 GAGAC GTCTC(1/5)
BsmI 524 GCATTC GAATGC(1/-1)
BspEI 413 TCCGGA T^CCGGA
BstYI 479 GGATCC [GA]^GATC[CT]
DrdI 490 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 483 CCACGG C^C[GA][CT]GG
EaeI 508 TGGCCA [CT]^GGCC[GA]
EarI 11 GAAGAG CTCTTC(1/4)
EcoRI 602 GAATTC G^AATTC
HapII 414 CCGG C^CGG
HgaI 595 GACGC GACGC(5/10)
HpaII 414 CCGG C^CGG
HphI 440 TCACC GGTGA(8/7)
Ksp632I 11 GAAGAG CTCTTC(1/4)
MaeI 815 CTAG C^TAG
MaeII 46 ACGT A^CGT
MflI 479 GGATCC [GA]^GATC[CT]
MluNI 508 TGGCCA TGG^CCA
MroI 413 TCCGGA T^CCGGA
MscI 508 TGGCCA TGG^CCA
MseI 712 TTAA T^TAA
MslI 532 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 80 CAGCGG C[AC]G^C[GT]G
MspI 414 CCGG C^CGG
PflMI 771 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 11 GAAGAGC GCTCTTC(1/4)
SspI 640 AATATT AAT^ATT
TfiI 707 GATTC G^A[AT]TC
Tru9I 712 TTAA T^TAA
Van91I 771 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 479 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 281 CCGC CCGC(-3/-1)
82 GCGG
AcsI 602 GAATTC [GA]^AATT[CT]
751 AAATTT
AcyI 45 GACGTC G[GA]^CG[CT]C
595 GACGCC
AluI 272 AGCT AG^CT
813 AGCT
AlwI 480 GATCC GGATC(4/5)
479 GGATC
ApoI 602 GAATTC [GA]^AATT[CT]
751 AAATTT
BpmI 587 CTCCAG CTGGAG(16/14)
634 CTGGAG
BsaHI 45 GACGTC G[GA]^CG[CT]C
595 GACGCC
BsmFI 488 GGGAC GGGAC(10/14)
325 GTCCC
DraII 182 AGGACCC [GA]G^G[ACGT]CC[CT]
405 AGGACCC
EcoO109I 182 AGGACCC [GA]G^G[ACGT]CC[CT]
405 AGGACCC
Hin1I 45 GACGTC G[GA]^CG[CT]C
595 GACGCC
PpuMI 182 AGGACCC [GA]G^G[AT]CC[CT]
405 AGGACCC
PstI 77 CTGCAG CTGCA^G
310 CTGCAG
SfcI 77 CTGCAG C^T[GA][CT]AG
310 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AvaII BbvI BmyI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TseI Tsp509I
TspRI