Restriction map for
pF1KB7770
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AvaII AviII
AvrII BanI BbeI BbvCI BcgI
BciVI BfaI BfrI BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MaeI MamI MluI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 404 TCCGGA T^CCGGA
AcsI 756 GAATTC [GA]^AATT[CT]
AflIII 1344 ACACGT A^C[GA][CT]GT
AhdI 1328 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1351 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 635 GGGCCC GGGCC^C
ApoI 756 GAATTC [GA]^AATT[CT]
AspEI 1328 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1252 GAGCTC G[AT]GC[AT]^C
BalI 830 TGGCCA TGG^CCA
BamHI 458 GGATCC G^GATCC
BbrPI 1345 CACGTG CAC^GTG
BclI 1364 TGATCA T^GATCA
BcnI 633 CCGGG CC^[GC]GG
BglI 24 GCCATTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1345 CACGTG [CT]AC^GT[GA]
BsaI 1184 GAGACC GGTCTC(1/5)
BsaWI 404 TCCGGA [AT]^CCGG[AT]
BseAI 404 TCCGGA T^CCGGA
BseRI 949 CTCCTC GAGGAG(10/8)
BsiHKAI 1252 GAGCTC G[AT]GC[AT]^C
BsmI 905 GCATTC GAATGC(1/-1)
BspEI 404 TCCGGA T^CCGGA
BsrBI 1399 CCGCTC CCGCTC(-3/-3)
BssSI 967 CACGAG CACGAG(-5/-1)
BstAPI 1350 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 635 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 830 TGGCCA [CT]^GGCC[GA]
Eam1105I 1328 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 298 GAAGAG CTCTTC(1/4)
EcoO109I 635 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 756 GAATTC G^AATTC
FbaI 1364 TGATCA T^GATCA
Ksp632I 298 GAAGAG CTCTTC(1/4)
MaeII 1346 ACGT A^CGT
MfeI 323 CAATTG C^AATTG
MluNI 830 TGGCCA TGG^CCA
MroI 404 TCCGGA T^CCGGA
MscI 830 TGGCCA TGG^CCA
MunI 323 CAATTG C^AATTG
NciI 633 CCGGG CC^[GC]GG
NcoI 5 CCATGG C^CATGG
PmaCI 1345 CACGTG CAC^GTG
PmlI 1345 CACGTG CAC^GTG
PshAI 1384 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1252 GAGCTC GAGCT^C
SapI 298 GAAGAGC GCTCTTC(1/4)
ScaI 488 AGTACT AGT^ACT
SspI 1155 AATATT AAT^ATT
TaqI 602 TCGA T^CGA
TfiI 612 GAATC G^A[AT]TC
TthHB8I 602 TCGA T^CGA
XcmI 308 CCAACTCCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 151 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1219 GAAGACATTC
BglII 587 AGATCT A^GATCT
1412 AGATCT
BsgI 877 GTGCAG GTGCAG(16/14)
1348 GTGCAG
BspHI 294 TCATGA T^CATGA
1040 TCATGA
BspMI 1235 ACCTGC ACCTGC(4/8)
1359 GCAGGT
EcoRV 97 GATATC GAT^ATC
598 GATATC
EcoT14I 5 CCATGG C^C[AT][AT]GG
1032 CCAAGG
FauI 678 CCCGC CCCGC(4/6)
1398 CCCGC
HapII 405 CCGG C^CGG
633 CCGG
HincII 10 GTTGAC GT[CT]^[GA]AC
118 GTTGAC
HindII 10 GTTGAC GT[CT]^[GA]AC
118 GTTGAC
HindIII 350 AAGCTT A^AGCTT
424 AAGCTT
HpaII 405 CCGG C^CGG
633 CCGG
MseI 449 TTAA T^TAA
763 TTAA
MspI 405 CCGG C^CGG
633 CCGG
RcaI 294 TCATGA T^CATGA
1040 TCATGA
SbfI 807 CCTGCAGG CCTGCA^GG
1404 CCTGCAGG
Sse8387I 807 CCTGCAGG CCTGCA^GG
1404 CCTGCAGG
StyI 5 CCATGG C^C[AT][AT]GG
1032 CCAAGG
Tru9I 449 TTAA T^TAA
763 TTAA
Tsp45I 1082 GTGAC ^GT[GC]AC
1336 GTCAC
XmnI 151 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1219 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp509I
TspRI XhoII