Restriction map for
pF1KE0204
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII Eam1105I EarI Eco81I EcoNI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I MamI
MboII MfeI MluI MluNI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 290 TCCGGA T^CCGGA
AcsI 313 GAATTC [GA]^AATT[CT]
Aor51HI 527 AGCGCT AGC^GCT
ApaI 483 GGGCCC GGGCC^C
ApoI 313 GAATTC [GA]^AATT[CT]
Asp700I 558 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 599 GGATCC G^GATCC
BanI 364 GGCACC G^G[CT][GA]CC
BbrPI 106 CACGTG CAC^GTG
BfaI 259 CTAG C^TAG
BpmI 400 CTGGAG CTGGAG(16/14)
BsaWI 290 TCCGGA [AT]^CCGG[AT]
BseAI 290 TCCGGA T^CCGGA
BseRI 82 GAGGAG GAGGAG(10/8)
BsgI 21 CTGCAC GTGCAG(16/14)
BsiEI 472 CGGCCG CG[GA][CT]^CG
BsmBI 307 GAGACG CGTCTC(1/5)
BspEI 290 TCCGGA T^CCGGA
BspHI 521 TCATGA T^CATGA
BsrI 182 CCAGT ACTGG(1/-1)
BssSI 346 CTCGTG CACGAG(-5/-1)
BstAPI 507 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 579 GGTGACC G^GT[ACGT]ACC
BstPI 579 GGTGACC G^GT[ACGT]ACC
BstYI 599 GGATCC [GA]^GATC[CT]
DdeI 431 CTAAG C^T[ACGT]AG
DrdI 582 GACCAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 472 CGGCCG [CT]^GGCC[GA]
EagI 472 CGGCCG C^GGCCG
EclXI 472 CGGCCG C^GGCCG
Eco47III 527 AGCGCT AGC^GCT
Eco52I 472 CGGCCG C^GGCCG
EcoO65I 579 GGTGACC G^GT[ACGT]ACC
EcoRI 313 GAATTC G^AATTC
EcoT14I 536 CCAAGG C^C[AT][AT]GG
HgaI 120 GACGC GACGC(5/10)
MaeI 259 CTAG C^TAG
MaeIII 580 GTGAC ^GT[ACGT]AC
MflI 599 GGATCC [GA]^GATC[CT]
MroI 290 TCCGGA T^CCGGA
NotI 471 GCGGCCGC GC^GGCCGC
PflMI 198 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 106 CACGTG CAC^GTG
PmlI 106 CACGTG CAC^GTG
PvuII 151 CAGCTG CAG^CTG
RcaI 521 TCATGA T^CATGA
ScaI 434 AGTACT AGT^ACT
SfaNI 101 GCATC GCATC(5/9)
SfcI 171 CTACAG C^T[GA][CT]AG
SmaI 494 CCCGGG CCC^GGG
StyI 536 CCAAGG C^C[AT][AT]GG
TaqI 389 TCGA T^CGA
Tsp45I 580 GTGAC ^GT[GC]AC
Tsp509I 314 AATT ^AATT
TthHB8I 389 TCGA T^CGA
Van91I 198 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 599 GGATCC [GA]^GATC[CT]
XmaCI 494 CCCGGG C^CCGGG
XmaI 494 CCCGGG C^CCGGG
XmnI 558 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 67 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
199 CAGGTCCTG
AvaI 128 CTCGGG C^[CT]CG[GA]G
494 CCCGGG
BanII 125 GGGCTC G[GA]GC[CT]^C
483 GGGCCC
BcnI 494 CCCGG CC^[GC]GG
495 CCGGG
BsaAI 106 CACGTG [CT]AC^GT[GA]
448 TACGTG
BsmAI 265 GAGAC GTCTC(1/5)
307 GAGAC
BsoBI 128 CTCGGG C^[CT]CG[GA]G
494 CCCGGG
CfoI 10 GCGC GCG^C
528 GCGC
DsaI 225 CCGCGG C^C[GA][CT]GG
475 CCGCGG
FauI 224 CCCGC CCCGC(4/6)
123 GCGGG
HaeII 9 AGCGCC [GA]GCGC^[CT]
527 AGCGCT
HhaI 10 GCGC GCG^C
528 GCGC
HinP1I 10 GCGC G^CGC
528 GCGC
HphI 579 GGTGA GGTGA(8/7)
458 TCACC
KspI 225 CCGCGG CCGC^GG
475 CCGCGG
MslI 102 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
573 CACGACGGTG
NciI 494 CCCGG CC^[GC]GG
495 CCGGG
NlaIII 327 CATG CATG^
522 CATG
PpuMI 200 AGGTCCT [GA]G^G[AT]CC[CT]
589 AGGTCCC
SacII 225 CCGCGG CCGC^GG
475 CCGCGG
TspRI 183 CAGTG CA[GC]TG[ACGT][ACGT]^
219 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MaeII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI