Restriction map for
pF1KE1497
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AspI AviII BbrPI
BbvCI BcgI BclI BfrI BglII
BlpI Bpu1102I BsiEI BsiWI Bsp1407I
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII Cfr10I CpoI DraII EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HgaI HpaI KpnI Ksp632I KspI
MluI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SmaI SpeI SphI Sse8387I
SspBI Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 2218 TCCGGA T^CCGGA
AcsI 1760 GAATTC [GA]^AATT[CT]
AcyI 1580 GGCGCC G[GA]^CG[CT]C
AhdI 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1057 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1166 AGCGCT AGC^GCT
ApaI 2415 GGGCCC GGGCC^C
ApoI 1760 GAATTC [GA]^AATT[CT]
AseI 610 ATTAAT AT^TAAT
AsnI 610 ATTAAT AT^TAAT
AspEI 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1331 CTCGAG C^[CT]CG[GA]G
AvrII 1965 CCTAGG C^CTAGG
BamHI 399 GGATCC G^GATCC
BbeI 1580 GGCGCC GGCGC^C
BciVI 2753 GTATCC GTATCC(6/5)
BlnI 1965 CCTAGG C^CTAGG
BsaAI 2750 TACGTA [CT]AC^GT[GA]
BsaHI 1580 GGCGCC G[GA]^CG[CT]C
BsaI 1559 GAGACC GGTCTC(1/5)
BsaWI 2218 TCCGGA [AT]^CCGG[AT]
BseAI 2218 TCCGGA T^CCGGA
BsmBI 1651 GAGACG CGTCTC(1/5)
BsmI 637 GAATGC GAATGC(1/-1)
BsoBI 1331 CTCGAG C^[CT]CG[GA]G
BspDI 2473 ATCGAT AT^CGAT
BspEI 2218 TCCGGA T^CCGGA
BstBI 1109 TTCGAA TT^CGAA
Cac8I 1122 GCGGGC GC[ACGT]^[ACGT]GC
ClaI 2473 ATCGAT AT^CGAT
DrdI 1976 GACTAGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1166 AGCGCT AGC^GCT
EcoRI 1760 GAATTC G^AATTC
EcoRV 448 GATATC GAT^ATC
Hin1I 1580 GGCGCC G[GA]^CG[CT]C
HincII 1099 GTTGAC GT[CT]^[GA]AC
HindII 1099 GTTGAC GT[CT]^[GA]AC
KasI 1580 GGCGCC G^GCGCC
MfeI 1229 CAATTG C^AATTG
MroI 2218 TCCGGA T^CCGGA
MunI 1229 CAATTG C^AATTG
NarI 1580 GGCGCC GG^CGCC
NcoI 326 CCATGG C^CATGG
NspI 2696 ACATGC [GA]CATG^[CT]
NspV 1109 TTCGAA TT^CGAA
PaeR7I 1331 CTCGAG C^TCGAG
PflMI 459 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1903 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 610 ATTAAT AT^TAAT
PvuII 2490 CAGCTG CAG^CTG
SacI 2798 GAGCTC GAGCT^C
SexAI 2151 ACCTGGT A^CC[AT]GGT
SfoI 1580 GGCGCC GGC^GCC
SfuI 1109 TTCGAA TT^CGAA
SnaBI 2750 TACGTA TAC^GTA
SspI 42 AATATT AAT^ATT
StuI 937 AGGCCT AGG^CCT
SwaI 2615 ATTTAAAT ATTT^AAAT
Van91I 459 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 798 TCTAGA T^CTAGA
XhoI 1331 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 343 GTAGAC GT^[AC][GT]AC
2830 GTCTAC
AfaI 981 GTAC GT^AC
2703 GTAC
AspHI 1794 GAGCAC G[AT]GC[AT]^C
2798 GAGCTC
BalI 248 TGGCCA TGG^CCA
2351 TGGCCA
BanI 1580 GGCGCC G^G[CT][GA]CC
2148 GGCACC
BanII 2415 GGGCCC G[GA]GC[CT]^C
2798 GAGCTC
BpmI 2449 CTCCAG CTGGAG(16/14)
1408 CTGGAG
BsgI 892 GTGCAG GTGCAG(16/14)
1438 GTGCAG
BsiHKAI 1794 GAGCAC G[AT]GC[AT]^C
2798 GAGCTC
BspHI 1022 TCATGA T^CATGA
2423 TCATGA
BsrBI 373 CCGCTC CCGCTC(-3/-3)
724 GAGCGG
BsrDI 1050 CATTGC GCAATG(2/0)
1181 CATTGC
BstAPI 2065 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2691 GCAGGACATGC
BstXI 1142 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1940 CCATGCAGATGG
DraI 1893 TTTAAA TTT^AAA
2616 TTTAAA
DraIII 1290 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2150 CACCTGGTG
DsaI 125 CCGTGG C^C[GA][CT]GG
326 CCATGG
HindIII 199 AAGCTT A^AGCTT
1401 AAGCTT
MaeII 158 ACGT A^CGT
2751 ACGT
MluNI 248 TGGCCA TGG^CCA
2351 TGGCCA
MscI 248 TGGCCA TGG^CCA
2351 TGGCCA
MspA1I 773 CAGCGG C[AC]G^C[GT]G
2490 CAGCTG
RcaI 1022 TCATGA T^CATGA
2423 TCATGA
RsaI 981 GTAC GT^AC
2703 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AluI AlwI AocI AvaII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaBI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I CfoI Cfr13I DdeI
DpnII EaeI Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII