##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AfaI          287  GTAC                 GT^AC
AlwNI         291  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          210  GGGCCC               GGGCC^C
BanII         210  GGGCCC               G[GA]GC[CT]^C
BcnI          165  CCGGG                CC^[GC]GG
BfaI          121  CTAG                 C^TAG
BmyI          210  GGGCCC               G[AGT]GC[ACT]^C
BsgI          218  GTGCAG               GTGCAG(16/14)
Bsp1286I      210  GGGCCC               G[AGT]GC[ACT]^C
BstF5I         11  GGATG                GGATG(2/0)
DraIII        100  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         214  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       236  CCTTGG               C^C[AT][AT]GG
FokI           11  GGATG                GGATG(9/13)
HaeII          33  GGCGCT               [GA]GCGC^[CT]
HapII         165  CCGG                 C^CGG
HpaII         165  CCGG                 C^CGG
MaeI          121  CTAG                 C^TAG
MboII         143  TCTTC                GAAGA(8/7)
MspA1I        310  CAGCTG               C[AC]G^C[GT]G
MspI          165  CCGG                 C^CGG
NciI          165  CCGGG                CC^[GC]GG
PvuII         310  CAGCTG               CAG^CTG
RsaI          287  GTAC                 GT^AC
SbfI          180  CCTGCAGG             CCTGCA^GG
SexAI         101  ACCTGGT              A^CC[AT]GGT
Sse8387I      180  CCTGCAGG             CCTGCA^GG
StyI          236  CCTTGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BpmI          244  CTGGAG               CTGGAG(16/14)
              313  CTGGAG              
BsmFI          51  GGGAC                GGGAC(10/14)
              252  GTCCC               
CfoI           15  GCGC                 GCG^C
               34  GCGC                
HhaI           15  GCGC                 GCG^C
               34  GCGC                
HinP1I         15  GCGC                 G^CGC
               34  GCGC                
SfaNI          12  GATGC                GCATC(5/9)
              293  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BspMI       BssKI       BstNI       
Cac8I       Cfr13I      DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      ItaI        MnlI        
MvaI        MwoI        NlaIV       PpuMI       PstI        
Sau96I      ScrFI       SfcI        TseI