Restriction map for
pF1KB6112
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BlnI BsaAI BseAI BsiEI
BsiWI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HpaI
KasI KpnI KspI MroI MspA1I
NaeI NarI NcoI NdeI NgoMIV
NotI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1423 GACGTC GACGT^C
AccI 1441 GTAGAC GT^[AC][GT]AC
AclI 1381 AACGTT AA^CGTT
AhdI 1507 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 747 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 768 CCTGAGG CC^T[ACGT]AGG
ApaI 473 GGGCCC GGGCC^C
AspEI 1507 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1056 TGGCCA TGG^CCA
BcgI 1736 GCATGAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 641 TGATCA T^GATCA
BglI 1049 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1792 AGATCT A^GATCT
BlpI 1292 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1292 GCTTAGC GC^T[ACGT]AGC
BsaWI 86 TCCGGT [AT]^CCGG[AT]
BsmBI 1375 CGTCTC CGTCTC(1/5)
Bsp1407I 830 TGTACA T^GTACA
BspLU11I 845 ACATGT A^CATGT
BsrBI 916 GAGCGG CCGCTC(-3/-3)
BsrGI 830 TGTACA T^GTACA
BstAPI 1164 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 768 CCTGAGG CC^T[ACGT]AGG
CelII 1292 GCTTAGC GC^T[ACGT]AGC
DrdI 233 GACCTAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1056 TGGCCA [CT]^GGCC[GA]
Eam1105I 1507 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 768 CCTGAGG CC^T[ACGT]AGG
EcoRV 558 GATATC GAT^ATC
EcoT14I 1823 CCAAGG C^C[AT][AT]GG
FbaI 641 TGATCA T^GATCA
HincII 523 GTCAAC GT[CT]^[GA]AC
HindII 523 GTCAAC GT[CT]^[GA]AC
HindIII 1330 AAGCTT A^AGCTT
MfeI 1076 CAATTG C^AATTG
MluNI 1056 TGGCCA TGG^CCA
MscI 1056 TGGCCA TGG^CCA
MunI 1076 CAATTG C^AATTG
NheI 1564 GCTAGC G^CTAGC
NruI 1023 TCGCGA TCG^CGA
NspI 845 ACATGT [GA]CATG^[CT]
PaeR7I 1744 CTCGAG C^TCGAG
PflFI 730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1381 AACGTT AA^CGTT
SapI 952 GAAGAGC GCTCTTC(1/4)
SmaI 1517 CCCGGG CCC^GGG
SspBI 830 TGTACA T^GTACA
StyI 1823 CCAAGG C^C[AT][AT]GG
Tsp45I 983 GTGAC ^GT[GC]AC
TspRI 429 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1744 CTCGAG C^TCGAG
XmaCI 1517 CCCGGG C^CCGGG
XmaI 1517 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 493 GACGCC G[GA]^CG[CT]C
1423 GACGTC
Asp700I 351 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1027 GAACGATTTC
AspHI 299 GAGCTC G[AT]GC[AT]^C
955 GAGCTC
BsaBI 262 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
558 GATATCTATC
BsaHI 493 GACGCC G[GA]^CG[CT]C
1423 GACGTC
BsaI 995 GAGACC GGTCTC(1/5)
1202 GAGACC
BseRI 302 CTCCTC GAGGAG(10/8)
314 GAGGAG
BsgI 1652 CTGCAC GTGCAG(16/14)
426 GTGCAG
BsiHKAI 299 GAGCTC G[AT]GC[AT]^C
955 GAGCTC
BspHI 644 TCATGA T^CATGA
1850 TCATGA
CfoI 1016 GCGC GCG^C
1451 GCGC
FauI 149 CCCGC CCCGC(4/6)
918 GCGGG
HaeII 1015 AGCGCC [GA]GCGC^[CT]
1450 AGCGCC
HgaI 493 GACGC GACGC(5/10)
1374 GCGTC
HhaI 1016 GCGC GCG^C
1451 GCGC
Hin1I 493 GACGCC G[GA]^CG[CT]C
1423 GACGTC
HinP1I 1016 GCGC G^CGC
1451 GCGC
MamI 262 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
558 GATATCTATC
MluI 42 ACGCGT A^CGCGT
1372 ACGCGT
MseI 47 TTAA T^TAA
1334 TTAA
MslI 31 CACGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1466 CATTGCTGTG
PpuMI 78 GGGTCCC [GA]G^G[AT]CC[CT]
340 GGGACCT
RcaI 644 TCATGA T^CATGA
1850 TCATGA
SacI 299 GAGCTC GAGCT^C
955 GAGCTC
ScaI 499 AGTACT AGT^ACT
1178 AGTACT
SfaNI 187 GATGC GCATC(5/9)
1222 GATGC
StuI 690 AGGCCT AGG^CCT
1802 AGGCCT
Tru9I 47 TTAA T^TAA
1334 TTAA
XmnI 351 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1027 GAACGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI TfiI TseI Tsp509I TthHB8I
XhoII